正常人淋巴细胞中糖皮质激素受体的昼夜节律和季节变化

Circadian and seasonal variations of glucocorticoid receptors in normal human lymphocytes.

作者信息

Schlaghecke R, Kley H K

出版信息

Steroids. 1986 Apr-May;47(4-5):287-94. doi: 10.1016/0039-128x(86)90098-x.

Abstract

Circulating human lymphocytes are known to contain specific glucocorticoid receptors. Using a competitive binding whole cell assay, we have examined the binding of [3H] dexamethasone to peripheral lymphocytes of normal male subjects. Lymphocytes were found to contain 2000-10000 glucocorticoid receptor sites/cell and the Kd value was in the range of 0.5-9 X 10(-9) M. The number and affinity of glucocorticoid receptors did not change throughout a 1-year observation time. In contrast, there was a significant diurnal variation in receptor content (38% higher at 11 p.m. than at 8 a.m.), while receptor affinity did not change.

摘要

已知循环中的人类淋巴细胞含有特定的糖皮质激素受体。我们采用竞争性结合全细胞分析法，检测了[3H]地塞米松与正常男性受试者外周淋巴细胞的结合情况。发现淋巴细胞每个细胞含有2000 - 10000个糖皮质激素受体位点，解离常数（Kd）值在0.5 - 9×10⁻⁹ M范围内。在1年的观察期内，糖皮质激素受体的数量和亲和力没有变化。相比之下，受体含量存在显著的昼夜变化（晚上11点比早上8点高38%），而受体亲和力没有改变。