Doll Sophie-Charlotte K, Haimerl Peggy, Bartel Alexander, Arlt Sebastian P
Clinic for Animal Reproduction Faculty of Veterinary Medicine Freie Universität Berlin Berlin Germany.
Institute for Veterinary Epidemiology and Biostatistics Faculty of Veterinary Medicine Freie Universität Berlin Berlin Germany.
Vet Rec Open. 2022 Jul 25;9(1):e40. doi: 10.1002/vro2.40. eCollection 2022 Dec.
Nonesterified fatty acids (NEFAs) are an important energy substrate in mammals. Measurement of the NEFA concentration in blood serum is common practice and enables reliable detection of a negative energy balance in several species. This parameter can be used to detect subclinical metabolic diseases or to optimise feeding to prevent severe negative energy balance. Since no reference values for dogs have been published, the aim of this study was to establish such values.
Blood serum from 85 healthy dogs was examined with a multiparameter clinical chemistry analyser. Given that NEFA values are not usually normally distributed, reference intervals (RIs) were calculated nonparametrically using bootstrapping (5000 replicates) for the 90% confidence intervals.
The examined cohort had a median age of 62.16 months (2-180 months) and a median weight of 19.2 kg (3.0-55.0 kg) and comprised 27 (31.8%) males and 58 (68.2%) females, with 32 (37.6%) neutered or spayed. The fasting time was 5.9 h (range 0-23 h). The tested confounders age, sex, neuter status, bodyweight and body condition score did not significantly affect the NEFA concentrations.
The NEFA RI for dogs in this study was 0.2-1.47 mmol/L. The results may be used to adjust food composition and amount in healthy dogs or to detect metabolic disorders. Further research on NEFA metabolism in dogs maintained in standardised conditions and in specific nutritional situations or with particular diseases is warranted.
非酯化脂肪酸（NEFAs）是哺乳动物重要的能量底物。检测血清中NEFA浓度是常见做法，能够可靠地检测多种物种的负能量平衡。该参数可用于检测亚临床代谢疾病或优化饲养以预防严重的负能量平衡。由于尚未公布犬类的参考值，本研究旨在确定这些值。
使用多参数临床化学分析仪检测85只健康犬的血清。鉴于NEFA值通常不呈正态分布，使用自助法（5000次重复）非参数计算90%置信区间的参考区间（RIs）。
受试队列的中位年龄为62.16个月（2 - 180个月），中位体重为19.2千克（3.0 - 55.0千克），包括27只（31.8%）雄性犬和58只（68.2%）雌性犬，其中32只（37.6%）已绝育。禁食时间为5.9小时（范围0 - 23小时）。受试的混杂因素年龄、性别、绝育状态、体重和身体状况评分对NEFA浓度无显著影响。
本研究中犬类的NEFA参考区间为0.2 - 1.47毫摩尔/升。该结果可用于调整健康犬的食物成分和量或检测代谢紊乱。有必要对处于标准化条件、特定营养状况或患有特定疾病的犬类的NEFA代谢进行进一步研究。
