School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, China.
International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China.
Food Funct. 2022 Aug 15;13(16):8662-8675. doi: 10.1039/d2fo00337f.
("Niu Dali" in Chinese) is a well-known edible plant in Southeast China. roots contain a high level of polysaccharides, which have been reported to show multiple health-promoting effects. In the current study, the anti-obesity effects of a crude extract of polysaccharides (NP) and its underlying mechanisms of action are investigated. C57BL/6 mice were divided into three groups and fed either a standard diet or a high-fat diet (HFD). The HFD + NP group mice received oral administration of NP (100 mg per kg per day) every other day for 10 weeks. NP supplementation alleviated HFD-induced diabetic biomarkers including body weight gain, hyperlipidemia, liver steatosis, and adipocyte hypertrophy. Western blot and RT-PCR analyses revealed that NP inhibited hepatic lipogenesis and adipogenesis ( decreased expression of Srebp1c, Fas, Cebpα, and Pparγ), stimulated adipocyte lipolysis (enhanced mRNA expression of and ), and attenuated HFD-induced hepatic inflammation (decreased expression of TNF-α and NF-κB p65). Furthermore, 16S rDNA and GC-MS analyses showed that NP supplementation restored the Firmicutes/Bacteroidetes proportion, elevated colon-derived SCFAs, especially acetic acid content, and increased the relative abundance of genera associated with SCFA production in HFD-fed mice. Findings from this study suggest that NP alleviated HFD-induced obesity in a mouse model, which was possibly due to its ameliorative effects on diet-induced gut dysbiosis. Polysaccharides from are promising prebiotics and they may be further developed as functional foods for the management of obesity.
（中文名为“牛大力”）是中国东南地区一种著名的食用植物。其根部含有高水平的多糖，据报道具有多种促进健康的作用。在本研究中，研究了多糖粗提物（NP）的抗肥胖作用及其潜在的作用机制。将 C57BL/6 小鼠分为三组，分别喂食标准饮食或高脂肪饮食（HFD）。HFD+NP 组小鼠每隔一天接受 NP（100mg/kg/天）口服给药，共 10 周。NP 补充缓解了 HFD 诱导的糖尿病生物标志物，包括体重增加、高血脂、肝脂肪变性和脂肪细胞肥大。Western blot 和 RT-PCR 分析表明，NP 抑制肝脂肪生成和脂肪生成（降低 Srebp1c、Fas、Cebpα 和 Pparγ 的表达），刺激脂肪细胞脂肪分解（增强 和 的 mRNA 表达），并减轻 HFD 诱导的肝炎症（降低 TNF-α 和 NF-κB p65 的表达）。此外，16S rDNA 和 GC-MS 分析表明，NP 补充恢复了厚壁菌门/拟杆菌门的比例，提高了结肠来源的 SCFAs，特别是乙酸含量，并增加了 HFD 喂养小鼠中与 SCFA 产生相关的属的相对丰度。本研究结果表明，NP 缓解了肥胖模型中的 HFD 诱导性肥胖，这可能是由于其对饮食诱导的肠道菌群失调的改善作用。来自 的多糖是有前途的益生元，它们可能被进一步开发为功能性食品，用于管理肥胖症。
