Vaxinano, Loos, France.
Univ. Lille, Inserm, CHU Lille, U1286-INFINITE-Institute for Translational Research in Inflammation, Lille, France.
PLoS One. 2022 Jul 29;17(7):e0272234. doi: 10.1371/journal.pone.0272234. eCollection 2022.
The nasal route of immunization has become a real alternative to injections. It is indeed described as more efficient at inducing immune protection, since it initiates both mucosal and systemic immunity, thus protecting against both the infection itself and the transmission of pathogens by the host. However, the use of immunomodulators should be limited since they induce inflammation. Here we investigated in vitro the mechanisms underlying the enhancement of antigen immunogenicity by starch nanoparticles (NPL) delivery systems in H292 epithelial cells, as well as the NPL's immunomodulatory effect. We observed that NPL had no intrinsic immunomodulatory effect but enhanced the immunogenicity of an E. coli lysate (Ag) merely by increasing its intracellular delivery. Moreover, we demonstrated the importance of the NPL density on their efficiency by comparing reticulated (NPL) and non-reticulated particles (NPL·NR). These results show that an efficient delivery system is sufficient to induce a mucosal immune response without the use of immunomodulators.
鼻内免疫途径已成为注射的真正替代方法。它确实被描述为更有效地诱导免疫保护，因为它可以同时诱导黏膜和全身免疫，从而既能预防感染本身，又能预防宿主传播病原体。然而，应限制使用免疫调节剂，因为它们会引起炎症。在这里，我们研究了淀粉纳米颗粒（NPL）递药系统在 H292 上皮细胞中增强抗原免疫原性的机制，以及 NPL 的免疫调节作用。我们观察到，NPL 本身没有固有免疫调节作用，但仅通过增加细胞内递送来增强大肠杆菌裂解物（Ag）的免疫原性。此外，我们通过比较网状（NPL）和非网状颗粒（NPL·NR），证明了 NPL 密度对其效率的重要性。这些结果表明，有效的递药系统足以在不使用免疫调节剂的情况下诱导黏膜免疫反应。
