College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong 266590, China.
College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong 266590, China; Institute of Yellow River Delta Earth Surface Processes and Ecological Integrity, Shandong University of Science and Technology Qingdao, Shandong 266590, China.
Bioresour Technol. 2022 Oct;361:127695. doi: 10.1016/j.biortech.2022.127695. Epub 2022 Jul 26.
Signaling molecules are useful in biofilm formation, but the mechanism for biofilm construction still needs to be explored. In this study, a signaling molecule, N-butyryl-l-Homoserine lactone (C-HSL), was supplied to enhance the construction of the sulfate-reducing bacteria (SRB) bio-cathode biofilm in microbial electrolysis cell (MEC). The sulfate reduction efficiency was more than 90% in less time under the system with C-HSL addition. The analysis of SRB bio-cathode biofilms indicated that the activity, distribution, microbial population, and secretion of extracellular polymers prompted by C-HSL, which accelerate the sulfate reduction, in particular for the assimilatory sulfate reduction pathway. Specifically, the relative abundance of acidogenic fermentation bacteria increased, and Desulfovibrio was co-metabolized with acidogenic fermentation bacteria. This knowledge will help to reveal the potential of signaling molecules to enhance the SRB bio-cathode biofilm MEC construction and improve the performance of treating sulfate-containing wastewater.
信号分子在生物膜形成中很有用，但生物膜构建的机制仍需探索。在这项研究中，添加了一种信号分子 N-丁酰基-L-高丝氨酸内酯（C-HSL），以增强硫酸盐还原菌（SRB）在微生物电解池（MEC）中生物阴极生物膜的构建。在添加 C-HSL 的系统中，硫酸盐还原效率在更短的时间内超过 90%。对 SRB 生物阴极生物膜的分析表明，C-HSL 促进了生物膜的活性、分布、微生物种群和胞外聚合物的分泌，从而加速了硫酸盐的还原，特别是对于同化硫酸盐还原途径。具体而言，产酸发酵细菌的相对丰度增加，脱硫弧菌与产酸发酵细菌共代谢。这一知识将有助于揭示信号分子在增强 SRB 生物阴极生物膜 MEC 构建和提高含硫酸盐废水处理性能方面的潜力。
