Yunker C E, Kuttler K L, Johnson L W
Vet Parasitol. 1987 Apr;24(1-2):7-13. doi: 10.1016/0304-4017(87)90125-7.
A virulent strain of Babesia bovis was adapted to grow in erythrocyte culture in the presence of equine serum and in lieu of bovine serum. Four splenectomized calves inoculated with the adapted strain, 429, developed hematologic signs of infection and a low grade fever, but remained free of central nervous system (CNS) signs and recovered. All of six control animals inoculated with a virulent strain reacted severely and five showed CNS signs and died. The calves injected with the attenuated strain were solidly immune when challenged with the virulent strain at 44 or 78 days after vaccination.
一种恶性牛巴贝斯虫毒株经驯化后，可在含有马血清而非牛血清的红细胞培养基中生长。四只接种了驯化毒株429的脾切除小牛出现了感染的血液学症状和低热，但未出现中枢神经系统（CNS）症状并康复。所有六只接种强毒株的对照动物反应严重，五只出现中枢神经系统症状并死亡。接种减毒株的小牛在接种后44天或78天用强毒株攻击时具有稳固的免疫力。
