Gorenflot A, Brasseur P, Precigout E, L'Hostis M, Marchand A, Schrevel J
Laboratoire d'Hématologie, U.F.R. Biologie Humaine et Expérimentale, Paris, France.
Parasitol Res. 1991;77(1):3-12. doi: 10.1007/BF00934377.
A continuous in vitro culture system for Babesia divergens was initiated from a human isolate. It was maintained through 305 subcultures for 3 years using a low concentration of serum and a low haematocrit, with no decrease in the initial virulence. This in vitro system enabled the routine culture of all human and bovine B. divergens isolates thus far tested, with a mean parasitaemia level of 30%-40%. Different cytological aspects observed in the same isolate by optical and electron microscopy were described in parasitized ox, gerbil and human erythrocytes. The sequence of B. divergens antibody responses was determined in man and ox, enabling the precise identification of major B. divergens antigens as candidates for vaccines.
一种用于双芽巴贝斯虫的连续体外培养系统是从一株人体分离株开始建立的。使用低浓度血清和低血细胞比容，该系统维持了3年，传代305次，初始毒力没有下降。这个体外系统能够对迄今为止测试的所有人体和牛的双芽巴贝斯虫分离株进行常规培养，平均寄生虫血症水平为30%-40%。通过光学显微镜和电子显微镜在感染的牛、沙鼠和人体红细胞中观察到同一分离株的不同细胞学特征。确定了人和牛体内双芽巴贝斯虫抗体反应的序列，从而能够精确鉴定作为疫苗候选物的主要双芽巴贝斯虫抗原。
