Plant Physiology and Biochemistry Research Laboratory, Department of Botany, UGC Centre for Advanced Study, The University of Burdwan, Burdwan, West Bengal, 713104, India.
Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Sci Rep. 2022 Jul 29;12(1):12989. doi: 10.1038/s41598-022-17078-2.
Regulation of oxidative stress towards origin of favorable internal redox cue plays a decisive role in salinity stress acclimation and least studied in rice and hence is the subject of present investigation. Redox landscaping of seedlings of ten experimental land races of rice of coastal Bangladesh grown under post imbibitional salinity stress (PISS) has been done through characterization of ROS-antioxidant interaction dynamics at metabolic interface, transcriptional reprogramming of redox-regulatory genes along with the assessment of biomarkers of oxidative threat for standardizing redox strategies and quality parameters for screening. The results exhibited a strong correlation between salinity induced redox status (pro-oxidant/antioxidant ratio, efficacy of HO turnover through integrated RboH-Ascorbate-Glutathione/Catalase pathway and estimation of sensitive redox biomarkers of oxidative deterioration) and germination phenotypes of all landraces of rice. Transcript abundance of the marker genes of the enzymes associated with central antioxidant hub for HO processing (CatA, OsAPx2, SodCc2, GRase and RboH) of all experimental landraces of the rice advocate the central role of HO turnover dynamics in regulating redox status and salinity tolerance. Landraces suffering greater loss of abilities of decisive regulation of HO turnover dynamics exhibited threat on the oxidative windows of the germinating seeds under salinity.
调控氧化应激以产生有利的内部氧化还原信号在盐胁迫适应中起着决定性作用，而这在水稻中研究最少，因此是本研究的主题。通过在吸胀后盐胁迫（PISS）下生长的孟加拉国沿海 10 个实验水稻地方品种幼苗的 ROS-抗氧化剂相互作用动力学的特征、氧化还原调节基因的转录重编程以及氧化胁迫生物标志物的评估，对幼苗进行了氧化还原景观处理，以标准化氧化还原策略和筛选质量参数。结果表明，盐诱导的氧化还原状态（促氧化剂/抗氧化剂比值、通过整合 RboH-抗坏血酸-谷胱甘肽/过氧化氢酶途径的 HO 周转率效率以及估计氧化恶化的敏感氧化还原生物标志物）与所有水稻地方品种的发芽表型之间存在很强的相关性。所有实验水稻地方品种中与 HO 加工相关的中央抗氧化枢纽酶的标记基因的转录丰度（CatA、OsAPx2、SodCc2、GRase 和 RboH）表明，HO 周转率动力学在调节氧化还原状态和耐盐性方面起着核心作用。在盐胁迫下，HO 周转率动力学的决定性调节能力丧失更大的地方品种对发芽种子的氧化窗产生威胁。
