Dey Ananya, Bhattacharjee Soumen
Plant Physiology and Biochemistry Research Laboratory, Department of Botany, UGC Centre for Advanced Study, The University of Burdwan, Burdwan, 713104 West Bengal India.
Physiol Mol Biol Plants. 2023 Apr;29(4):471-493. doi: 10.1007/s12298-023-01303-x. Epub 2023 Apr 6.
In the present investigation we have manipulated seeds of two indica rice cultivars, differing in sensitivity towards salinity stress ( L. cv. IR29 and Pokkali), with different combination of germination influencing hormones and redox modulating agents [500 µM Gibberellic acid (GA) + 20 mM HO, 500 µM GA + 100 µM Diphenyleneiodonium chloride (DPI), 500 µM GA + 500 µM N,N-dimethylthiourea (DMTU), 30 µM Triadimefon (TDM) + 100 µM DPI, 30 µM TDM + 500 µM DMTU] during early imbibition for exploring significance of regulation of oxidative window during germination. Reactive oxygen species (ROS)-antioxidant (AOX) interaction dynamics, assessed through redox metabolic fingerprints revealed significant changes in oxidative window of germinating tissue under redox and hormonal priming. GA (500 µM) + HO (20 mM) priming formed favorable redox cue and opened the oxidative window for germination, whereas GA (500 µM) + DPI (100 µM), GA (500 µM) + DMTU (500 µM) and TDM (30 µM) + DPI (100 µM) combination failed to generate redox cue for opening the oxidative window at metabolic interface. Assessment of transcript abundance of genes of enzymes of central redox hub (RBOH-SOD-ASC-GSH/CAT pathway) further confirmed the transcriptional reprogramming of genes () necessary for antioxidant-coupled origin of redox cue for germination. Assessment of pool of gibberellic acid, abscisic acid and jasmonic acid revealed a close connection between the hormonal homeostasis and internal redox cue. Role of oxidative window generated during metabolic reactivation phase for successful progression of germination is suggested.
The online version contains supplementary material available at 10.1007/s12298-023-01303-x.
在本研究中，我们用不同组合的发芽影响激素和氧化还原调节剂 [500 μM 赤霉素 (GA) + 20 mM 过氧化氢 (HO)、500 μM GA + 100 μM 二苯基碘鎓氯化物 (DPI)、500 μM GA + 500 μM N,N - 二甲基硫脲 (DMTU)、30 μM 三唑酮 (TDM) + 100 μM DPI、30 μM TDM + 500 μM DMTU] 处理了两个对盐胁迫敏感性不同的籼稻品种（IR29 品种和 Pokkali 品种）的种子，在早期吸胀阶段探索发芽过程中氧化窗口调控的重要性。通过氧化还原代谢指纹评估的活性氧 (ROS)-抗氧化剂 (AOX) 相互作用动态揭示了在氧化还原和激素引发下萌发组织氧化窗口的显著变化。GA (500 μM) + HO (20 mM) 引发形成了有利的氧化还原信号并打开了发芽的氧化窗口，而 GA (500 μM) + DPI (100 μM)、GA (500 μM) + DMTU (500 μM) 和 TDM (30 μM) + DPI (100 μM) 组合未能在代谢界面产生打开氧化窗口的氧化还原信号。对中心氧化还原枢纽（呼吸爆发氧化酶同源蛋白 - 超氧化物歧化酶 - 抗坏血酸 - 谷胱甘肽/过氧化氢酶途径）酶基因转录丰度的评估进一步证实了发芽中抗氧化剂偶联氧化还原信号起源所需基因的转录重编程。对赤霉素、脱落酸和茉莉酸库的评估揭示了激素稳态与内部氧化还原信号之间的密切联系。提示了代谢重新激活阶段产生的氧化窗口对发芽成功进展的作用。
在线版本包含可在 10.1007/s12298 - 023 - 01303 - x 获得的补充材料。
