KOPI：激酶抑制剂或蛋白质组影响分析。
KOPI: Kinase inhibitOr Proteome Impact analysis.
机构信息
Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA.
出版信息
Sci Rep. 2022 Jul 29;12(1):13015. doi: 10.1038/s41598-022-16557-w.
Kinase inhibitors often exert on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI's utility with staurosporine (STS) on the leukemic K562 cell proteome. We identified systematically staurosporine's non-kinome interactors, and showed for the first time that it caused paradoxical hyper- and biphasic phosphorylation.
激酶抑制剂通常会产生脱靶效应，因此高效的数据分析对于评估其对蛋白质组的影响至关重要。我们开发了一种快速进行这种蛋白质组评估的工作流程，称为激酶抑制剂蛋白质组影响分析 (KOPI)。我们用 STS 对白血病 K562 细胞蛋白质组进行了 KOPI 的实用性验证。我们系统地鉴定了 STS 的非激酶相互作用物，并首次表明它会导致反常的超磷酸化和双相磷酸化。
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