Biochemical Engineering Group, INCITAA, CIC, CONICET, Ingeniering School, Mar del Plata National University, Av Juan B Justo 4302, Mar del Plata B7608FDQ, Argentina E-mail:
CIDCA, CONICET, CIC, La Plata National University, 47 y 116, La Plata B1900AJJ, Argentina.
Water Sci Technol. 2022 Jul;86(2):292-301. doi: 10.2166/wst.2022.204.
In this work a parametric study and a bench bioreactor degradation test of Direct Black 22 (DB22) by Penicillium chrysogenum was performed as a first approach to an industrial application, framed within a policy of sustainable processes development. Three ancillary carbon sources and their optimum initial concentrations were studied. These were: glucose, potato starch and potato industry wastewater. Their optimum initial concentration was 6 g/L. The use of potato starch as co-substrate showed the highest decolorization rate and COD removal. Degradation of DB22 using different immobilization supports (stainless steel sponge, loofah sponge and polyethylene strips) was studied and the results showed that the time needed for the treatment decreased from 6 to 4 d. Phytotoxicity was evaluated in the final products of the immobilized cells assays, using Lactuca sativa seeds. For all treatments phytoxicity was reduced with respect to the untreated wastewater, except for the assays using polyethylene strips. Finally, the reuse of the biomass attached to different carriers and the performance of the treatment of DB22 in a 1 L bench scale bioreactor were tested. P. chrysogenum decolorized at least four sucesives reuses. The reactor assays showed a better performance of the treatment.
本工作采用产黄青霉（Penicillium chrysogenum）对直接黑 22（DB22）进行了参数研究和台式生物反应器降解试验，作为工业应用的初步尝试，符合可持续工艺发展政策。研究了三种辅助碳源及其最佳初始浓度。这些是：葡萄糖、土豆淀粉和土豆工业废水。它们的最佳初始浓度为 6 g/L。使用土豆淀粉作为共底物显示出最高的脱色率和 COD 去除率。研究了不同固定化载体（不锈钢海绵、丝瓜络海绵和聚乙烯条）对 DB22 的降解作用，结果表明，处理所需的时间从 6 天缩短到 4 天。使用莴苣种子评估了固定化细胞试验最终产物的植物毒性。对于所有处理，与未处理的废水相比，植物毒性都降低了，除了使用聚乙烯条的试验。最后，测试了不同载体上附着的生物量的再利用以及在 1 L 台式生物反应器中处理 DB22 的性能。产黄青霉至少进行了四次重复利用脱色。反应器试验表明处理效果更好。
