Division of Biomedical Engineering, Department of Life science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439957131, Iran.
Division of Biomedical Engineering, Department of Life science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439957131, Iran.
Colloids Surf B Biointerfaces. 2022 Oct;218:112729. doi: 10.1016/j.colsurfb.2022.112729. Epub 2022 Jul 26.
This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method. Undoped and Cu-doped ZnO (ZnCuO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques. The XRD evaluation determined that the concentration of copper in ZnO is limited to below 5%. Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains. The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (156 µm) with high water absorption (3089%) and proper elongation (~49%) in a wet medium. The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge. Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells. Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
本研究旨在通过将掺铜氧化锌纳米粒子（ZnCuO，x=0.03 和 0.05）引入到通过冻干法制备的羧甲基纤维素/明胶戊二醛交联复合海绵中，开发出一种新型的抗菌超吸水性敷料。采用稳定剂辅助沉淀法合成了未掺杂和掺铜氧化锌纳米粒子，并通过 XRD、FESEM、FTIR 和 ICP-OES 技术进行了表征。XRD 评估确定氧化锌中的铜浓度限制在 5%以下。此外，ICP-OES 分析证实了掺杂过程对 ZnO 晶体结构的影响，因为掺铜 ZnO 释放出更多的锌和铜离子，从而提高了对金黄色葡萄球菌和大肠杆菌菌株的抗菌活性。氧化锌纳米粒子对优化复合海绵物理和机械性能的影响表明，掺入 3wt%的氧化锌纳米粒子可在湿介质中产生具有高吸水性（3089%）和适当伸长率（49%）的良好互联多孔结构（~156μm）。掺铜氧化锌纳米粒子增强了复合海绵的抗菌潜力。同时，所有海绵组对人真皮成纤维细胞的活力、增殖和黏附都是安全的。总的来说，获得的数据证明了羧甲基纤维素/明胶/掺铜氧化锌敷料作为一种有前途的感染性伤口管理候选物的潜力。
