Department of Rheumatology and Immunology, The People's Hospital of Suzhou New District, Suzhou, 215000, China.
College of Chemistry and Pharmacy, Northwest A&F University, No.22 Xinong Road, Yangling, 712100, Shaanxi, China.
Sci Rep. 2022 Jul 30;12(1):13115. doi: 10.1038/s41598-022-17531-2.
The lipid composition performs important functions in interaction between macropha-ge and Mycobacterium tuberculosis (MTB)/Mycobacterium bovis (MB). Current understanding regarding the lipid responses of bovine alveolar macrophage (BAM) to MTB/MB is quite limited. The present study conducted lipidomics and transcriptome to assess alterations in BAM lipid compositions upon MB and MTB infection. We found that both MTB and MB induced glycerophospholipids accumulation in BAM, and MTB induced more alterations in lipid composition. MTB could affect the contents of various lipids, especially ceramide phosphocholines, polystyrene (PS) (17:0/0:0), testolic acid and testosterone acetate. Meanwhile, MB particularly induced accumulation of 1-alkyl,2-acylglycerophosphoinositols. Both MB and MTB suppressed the contents of palmitoleamide, N-ethyl arachidonoyl amine, N-(1,1-dimethyl-2-hydroxy-ethyl) arachidonoyll amine, eicosanoyl-EA, and PS (O-18:0/17:0) in BAM. Additionally, transcriptome analysis revealed that only MTB triggered genes involved in immune signaling and lipid related pathways in BAM. And MTB mainly activated genes CXCL2 and CXCL3 relevant to NOD-like receptor, IL-17 and TNF to further induce lipid accumulation in BAM, which in turn promoted the formation of foam cells. Meanwhile, time course RT-qPCR results showed that MTB was recognized by BAM to triggered dramatic immune responses, whereas MB could effectively escape the recognition system of BAM, leading rearrangement of lipid metabolisms in BAM at early infection stage. Altogether, the results of the present study provided evidence for changes in lipid metabolism of MTB/MB attacked BAM and contributed to the detection and treatment of zoonotic tuberculosis.
脂质组成在巨嗜细胞与结核分枝杆菌（MTB）/牛分枝杆菌（MB）之间的相互作用中发挥着重要功能。目前对于牛肺泡巨噬细胞（BAM）对 MTB/MB 的脂质反应的了解相当有限。本研究通过脂质组学和转录组学评估了 MB 和 MTB 感染后 BAM 脂质组成的变化。我们发现 MTB 和 MB 均可诱导 BAM 中甘油磷脂的积累，而 MTB 引起的脂质组成变化更多。MTB 可以影响各种脂质的含量，特别是神经酰胺磷酸胆碱、聚苯乙烯（PS）（17:0/0:0）、testolic 酸和睾丸酮醋酸酯。同时，MB 特别诱导 1-烷基、2-酰基甘油磷酸肌醇的积累。MB 和 MTB 均抑制 BAM 中棕榈油酸酰胺、N-乙基花生四烯酰胺、N-（1,1-二甲基-2-羟基-乙基）花生四烯酰基胺、二十碳烯酰-EA 和 PS（O-18:0/17:0）的含量。此外，转录组分析显示，只有 MTB 触发了 BAM 中涉及免疫信号和脂质相关途径的基因。MTB 主要激活与 NOD 样受体、IL-17 和 TNF 相关的 CXCL2 和 CXCL3 基因，进一步诱导 BAM 中脂质的积累，从而促进泡沫细胞的形成。同时，时间过程 RT-qPCR 结果表明，MTB 被 BAM 识别，引发强烈的免疫反应，而 MB 可以有效地逃避 BAM 的识别系统，导致早期感染阶段 BAM 中脂质代谢的重新排列。总之，本研究结果为 MTB/MB 攻击 BAM 时脂质代谢的变化提供了证据，并有助于检测和治疗人畜共患结核病。
