College of Chemistry and Chemical Engineering, Xi'an Shiyou University, Xi'an, 710065, China.
Beijing National Laboratory for Molecular Sciences, CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Small. 2022 Aug;18(34):e2202405. doi: 10.1002/smll.202202405. Epub 2022 Jul 30.
The development of high-efficiency enzyme mimics is of great significance in the field of biocatalysis. However, it remains challenging to design novel enzyme mimics with multiple enzyme-like activities, excellent stability, and good reusability. Herein, a facile molecular assembly strategy to construct dialdehyde cellulose (DAC) templated Cu-doped polydopamine (DAC@PDA/Cu) membrane with dual enzyme-like activities is presented. The Schiff base bonds formed between polydopamine (PDA) and DAC can not only accelerate the adhesion of PDA thin layer but also contribute to Cu-loading and high stability of DAC@PDA/Cu membrane. Importantly, the assembled DAC@PDA/Cu membrane exhibits a remarkable catalytic activity that is superior to the natural laccase along with high stability and excellent reusability. Moreover, the DAC@PDA/Cu membrane also demonstrates peroxidase-like activity, and it is successfully applied in the sensitive detection of ascorbic acid (AA). This work will provide a new paradigm methodology for rational design and practical applications of enzyme mimics based on bioinspired molecular assemblies.
高效酶模拟物的开发在生物催化领域具有重要意义。然而，设计具有多种酶样活性、优异的稳定性和良好的可重复使用性的新型酶模拟物仍然具有挑战性。本文提出了一种简便的分子组装策略，用于构建具有双酶样活性的二醛纤维素（DAC）模板化铜掺杂聚多巴胺（DAC@PDA/Cu）膜。聚多巴胺（PDA）和 DAC 之间形成的席夫碱键不仅可以加速 PDA 薄层的附着，而且有助于 Cu 负载和 DAC@PDA/Cu 膜的高稳定性。重要的是，组装的 DAC@PDA/Cu 膜表现出优异的催化活性，优于天然漆酶，同时具有高稳定性和优异的可重复使用性。此外，DAC@PDA/Cu 膜还表现出过氧化物酶样活性，并成功应用于抗坏血酸（AA）的灵敏检测。这项工作将为基于生物启发分子组装的酶模拟物的合理设计和实际应用提供一种新的范例方法。
