Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology (Institute of Animal Genetics and Breeding), Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Ecotoxicol Environ Saf. 2022 Sep 1;242:113924. doi: 10.1016/j.ecoenv.2022.113924. Epub 2022 Jul 28.
Thiram pollution is one of the main causes of tibial dyschondroplasia (TD) induced by feed sources. Several studies have speculated that miRNA, circRNA and lncRNA may have significant impact on the development of TD, however, the specific mRNAs and noncoding RNAs and their respective regulatory mechanisms and functions in the development of TD have not been explored. Therefore, in this present study, we screened the differentially expressed mRNA, miRNA, circRNA and lncRNA by whole-transcriptome sequencing (RNA-seq) and differentially expressed genes (DEGs) enrichment, as well as constructed the interaction network among the mRNA-miRNA, mRNA-lncRNA and mRNA-miRNA-circRNA. The sequencing results were verified by fluorescence real-time quantitative PCR (RT-qPCR). The results obtained in this study, revealed that the cells were atrophied and disordered in the TD group, and the expression of BMP6, TGF-β and VEGF were significantly reduced. A total of 141 mRNAs, 10 miRNAs, 23 lncRNAs and 35 circRNAs of DEGs were obtained (p<0.05) Theses DEGs were enriched in the adhere junction and insulin signaling pathways. In addition, the mRNA-miRNA-circRNA network suggested that several pivotal ceRNA showed a regulatory relationship between the transcripts with miRNA, circRNA or lncRNA. Taken together, the results in the present study, represent an insight for further functional research on the ceRNA regulatory mechanism of TD in broilers.
福美双污染是饲料源诱发胫骨软骨发育不良（TD）的主要原因之一。有几项研究推测，miRNA、circRNA 和 lncRNA 可能对 TD 的发生发展有重要影响，但 TD 发生发展过程中特定的 mRNA 和非编码 RNA 及其各自的调控机制和功能尚未被探索。因此，在本研究中，我们通过全转录组测序（RNA-seq）筛选差异表达的 mRNA、miRNA、circRNA 和 lncRNA，进行差异表达基因（DEGs）富集，并构建了 mRNA-miRNA、mRNA-lncRNA 和 mRNA-miRNA-circRNA 之间的相互作用网络。荧光实时定量 PCR（RT-qPCR）验证了测序结果。本研究结果表明，TD 组细胞萎缩、排列紊乱，BMP6、TGF-β和 VEGF 表达明显降低。获得了 141 个差异表达的 mRNAs、10 个 miRNAs、23 个 lncRNAs 和 35 个 circRNAs（p<0.05）。这些差异表达基因在黏附连接和胰岛素信号通路中富集。此外，mRNA-miRNA-circRNA 网络表明，一些关键的 ceRNA 与 miRNA、circRNA 或 lncRNA 的转录物之间存在调节关系。总之，本研究的结果为进一步研究肉鸡 ceRNA 调控机制提供了新的见解。
