Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Parasitology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Department of Parasitology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Medicinal and Applied Chemistry, College of Life Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Acta Trop. 2022 Nov;235:106580. doi: 10.1016/j.actatropica.2022.106580. Epub 2022 Jul 28.
Hymenolepis nana, a parasitic tapeworm distributed worldwide, is very prevalent in countries with poor sanitary conditions. Garlic is widely used as a seasoning and medicinal plant all over the world, and its derivatives have proven anti-microbial and anti-inflammatory effects. Our study explored the cestocidal and therapeutic effects of allicin derivatives against H. nana in vitro and in vivo. Worms taken from a host were cultured in vitro, and the effects of allyl sulfide (DAS), allyl disulfide (DADS) and dimethyl sulfoxide (DMSO) treatments were observed. Male BALB/c mice were then fed eggs to produce infection, given drugs for ten days and dissected. The results of this study showed that DADS in garlic exhibited good cestocidal effects in vitro and in vivo. DADS and DATS reduced motility, induced mortality and damaged body segments of worms in vitro. In vivo, the number of worms in the low-dose and high-dose DADS groups was significantly less than the infected control group. DADS effected cytokine changes in BALB/c mice after infection. IFN-γ increased, IL-2, 4, 6 and 13 decreased, and IL-5, 10 and IL-12 p70 did not change significantly. As a medicinal plant, garlic has many active ingredients that can developed as anti-microbial or parasite-related drugs.
微小膜壳绦虫是一种分布于世界各地的寄生绦虫，在卫生条件较差的国家非常普遍。大蒜作为一种调味品和药用植物，在世界各地广泛应用，其衍生物已被证明具有抗微生物和抗炎作用。我们的研究探讨了大蒜衍生的大蒜素对微小膜壳绦虫的杀绦虫和治疗作用，无论是在体外还是体内。从宿主中取出的蠕虫在体外进行培养，并观察丙烯基硫醚 (DAS)、丙烯基二硫化物 (DADS) 和二甲基亚砜 (DMSO) 处理的效果。然后，雄性 BALB/c 小鼠喂食虫卵以产生感染，给予药物治疗十天并进行解剖。本研究结果表明，大蒜中的 DADS 具有良好的体外和体内杀绦虫作用。DADS 和 DATS 在体外降低了蠕虫的运动性，诱导了其死亡率并破坏了其身体节段。在体内，低剂量和高剂量 DADS 组的蠕虫数量明显少于感染对照组。DADS 影响感染后 BALB/c 小鼠的细胞因子变化。IFN-γ 增加，IL-2、4、6 和 13 减少，IL-5、10 和 IL-12 p70 没有明显变化。作为一种药用植物，大蒜含有许多活性成分，可开发为抗微生物或寄生虫相关药物。
