Department of Morphological Sciences, College of Medical Sciences, Institute of Medical Sciences, University of Rzeszów, Rzeszów, Poland.
Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Lublin, Poland.
Brain Res. 2022 Oct 1;1792:148036. doi: 10.1016/j.brainres.2022.148036. Epub 2022 Jul 29.
There is little information about oligodendrocytes (OLGs) in the Periaqueductal Gray matter (PAG). The literature has not provided data on the number, morphology, or quantification of the expression of the OLG protein yet. Myelin Basic Protein (MBP) in this region of the Central Nervous system (CNS). The study aimed was to perform a comparative analysis: the location and morphology of OLGs, the cellular and regional distribution of iron, and the number of OLGs in PAG and corpus callosum (CC) of adult 16 male and 16 female sheep. To determine the location of the OLG of PAG and CC, the method of impregnation of the neuroglia with silver salts was applied. In turn, the Nissl method was used to determine the location of the brain structure and to analyse the number of OLG. The performed analysis showed that PAG, OLGs are located singly or in pairs in blood vessels and neurons, while in CC they are arranged in characteristic rows and accompany both nerve fibres and blood vessels. Immunofluorescent staining for the presence of MBP confirmed the location of OLGs in male and female sheep. Morphometric analysis showed the importance of these glial cells in OLG-myelin fibres, correlation in adults regardless of sex even after the creation of the completion of myelin. The results obtained indicate that the functions of OLGs are not only confined to myelination in young individuals, but also play a crucial role in the brain of adults. Our observations seem to be useful to better understanding OLGs biology.
关于少突胶质细胞（OLGs）在导水管周围灰质（PAG）中的信息很少。文献尚未提供该区域少突胶质细胞蛋白表达的数量、形态或定量数据。本研究旨在进行对比分析：OLGs 的位置和形态、铁的细胞和区域分布以及成年 16 只雄性和 16 只雌性绵羊 PAG 和胼胝体（CC）中的 OLG 数量。为了确定 PAG 和 CC 中 OLG 的位置，应用了神经胶质银盐浸渍法。反过来，尼氏染色法用于确定脑结构的位置并分析 OLG 的数量。进行的分析表明，PAG 中的 OLG 单独或成对位于血管和神经元中，而在 CC 中，它们呈特征性排列，伴随神经纤维和血管。存在 MBP 的免疫荧光染色证实了雄性和雌性绵羊 OLG 中的位置。形态计量学分析表明，这些神经胶质细胞在 OLG-髓鞘纤维中的重要性，在成年个体中无论性别如何都存在相关性，即使在髓鞘完成后也是如此。研究结果表明，OLGs 的功能不仅局限于年轻个体的髓鞘形成，而且在成年大脑中也起着至关重要的作用。我们的观察似乎有助于更好地了解 OLGs 的生物学。
