在 CO2 富集条件下，利用食品加工废物等可再生底物培养海洋微藻球等鞭金藻生产二十二碳六烯酸。
Docosahexaenoic acid production of the marine microalga Isochrysis galbana cultivated on renewable substrates from food processing waste under CO enrichment.
机构信息
College of Food Science and Technology, MOE Biomass Energy Research Center, State Key Laboratory of Food Science and Technology, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi 330047, People's Republic of China.
College of Food Science and Technology, MOE Biomass Energy Research Center, State Key Laboratory of Food Science and Technology, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi 330047, People's Republic of China.
出版信息
Sci Total Environ. 2022 Nov 20;848:157654. doi: 10.1016/j.scitotenv.2022.157654. Epub 2022 Jul 28.
Leftover dough is a starch-rich food processing waste of Chinese steamed bread. Leftover dough hydrolysates enriched with glucose and amino acids were used to cultivate the marine microalga Isochrysis galbana to produce docosahexaenoic acid (DHA) under CO enrichment. Isochrysis galbana could use mixed carbon sources (CO, glucose, and amino acids) synchronously to grow and accumulate DHA. Cell growth, the uptake of glucose and amino acids, and DHA production were significantly affected by CO enrichment. The maximum biomass concentration of 3.85 g L was achieved with 3 % CO. And the maximum DHA yield was 65.5 mg L d. To enhance DHA production, a two-stage cultivation strategy was successfully developed by this work. The maximum DHA yield of the two-stage culture was elevated by 2.3-fold. It is feasible to produce DHA by Isochrysis galbana using leftover dough under CO enrichment.
剩余面团是中国馒头生产过程中富含淀粉的加工废弃物。利用富含葡萄糖和氨基酸的剩余面团水解产物，在 CO2 富集条件下培养海洋微藻球等鞭金藻生产二十二碳六烯酸（DHA）。球等鞭金藻可以同步利用混合碳源（CO2、葡萄糖和氨基酸）进行生长和 DHA 积累。CO2 富集对细胞生长、葡萄糖和氨基酸的摄取以及 DHA 的生产有显著影响。在 3%的 CO2 条件下，最大生物量浓度达到 3.85 g/L，最大 DHA 产量为 65.5 mg/L/d。为了提高 DHA 的产量，本研究采用两段式培养策略，成功提高了 DHA 的产量。两段式培养的最大 DHA 产量提高了 2.3 倍。利用 CO2 富集剩余面团生产 DHA 是可行的。
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