Qu Yan, Bao Guozhang, Pan Xinyu, Bao Lan, Guo Jiancai, Xi Jinghui, Zhang Xin, Yang Yinan, Zhao Hongwei, Li Guomei
Key Laboratory of Groundwater Resources and Environment of the Ministry of Education (Jilin University), Changchun 130012, China; and Jilin Provincial Key Laboratory of Water Resources and Environment, Changchun 130012, China; and College of New Energy and Environment, Jilin University, Changchun 130012, China.
Key Laboratory for Vegetation Ecology, Ministry of Education, Institute of Natural Disaster Research, School of Environment, Northeast Normal University, Changchun 130024, China.
Funct Plant Biol. 2022 Oct;49(11):958-969. doi: 10.1071/FP21359.
In Qinghai-Tibet Plateau, crops are commonly subjected to freeze-thaw and salt stress factors simultaneously, and allelopathy is common, which affects the growth of highland barley (Hordeum vulgare L.), the largest food crop in Tibet. In order to explore the effects of artemisinin, salt and freeze-thaw (FAS) stress on physiological characteristics of highland barley seedlings, hydroponic experiment was carried out with the addition of 20mg/L artemisinin and 150mMNaCl as well as the simulation of freeze-thaw environment. The results suggested that under combined stress, the soluble protein content in combined stresses of artemisinin, FAS increased by 97.8%, the variation of relative conductivity in FAS group was lower than that in combined salt and freeze-thaw stress (FS), the relative water content decreased significantly (P <0.05), the malondialdehyde (MDA), H2 O2 and soluble sugar content in FAS group accumulated but less than those in FS group, and the superoxide dismutase (SOD) activity in combined artemisinin and freeze-thaw stress (FA) and FAS groups decreased. In addition, after freeze-thaw treatment, photosynthesis was weakened, and internal CO2 conentration (C i ) in FAS group significantly decreased (P <0.05). This study proved that appropriate amount of artemisinin can alleviate the damage of salt and freeze-thaw stress on barley seedlings.
在青藏高原，作物通常同时受到冻融和盐胁迫因素影响，化感作用普遍存在，这影响了西藏最大的粮食作物青稞（Hordeum vulgare L.）的生长。为了探究青蒿素、盐和冻融（FAS）胁迫对青稞幼苗生理特性的影响，开展了水培实验，添加20mg/L青蒿素和150mM NaCl，并模拟冻融环境。结果表明，在复合胁迫下，青蒿素与FAS复合胁迫下可溶性蛋白含量增加了97.8%，FAS组相对电导率变化低于盐与冻融复合胁迫（FS）组，相对含水量显著降低（P<0.05），FAS组丙二醛（MDA）、H2O2和可溶性糖含量积累但低于FS组，青蒿素与冻融复合胁迫（FA）组和FAS组超氧化物歧化酶（SOD）活性降低。此外，冻融处理后光合作用减弱，FAS组内部CO2浓度（Ci）显著降低（P<0.05）。本研究证明适量的青蒿素可减轻盐和冻融胁迫对大麦幼苗的伤害。
