蛋白融合策略通过 cryo-EM 促进小膜蛋白的结构测定。

The Protein Fusion Strategy Facilitates the Structure Determination of Small Membrane Proteins by Cryo-EM.

机构信息

State Key Laboratory of Membrane Biology, College of Future Technology, Institute of Molecular Medicine, Peking University, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Beijing 100871, China.

Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

出版信息

Biochemistry. 2023 Jan 17;62(2):196-200. doi: 10.1021/acs.biochem.2c00319. Epub 2022 Aug 1.

DOI:10.1021/acs.biochem.2c00319

PMID:35909370

Abstract

Despite the resolution revolution of cryo-EM, structures of small membrane proteins (<80 kDa) are still understudied. These proteins are notoriously reluctant to structure determination by single-particle cryo-EM. Protein fusion might represent a plausible strategy to overcome such difficulties. This Perspective enumerates recent exemplary progress and discusses the future potential of the protein fusion strategy.

摘要

尽管 cryo-EM 分辨率革命取得了突破，但<80 kDa 的小膜蛋白结构仍然研究不足。这些蛋白通过单颗粒 cryo-EM 进行结构测定非常困难。蛋白融合可能是克服这些困难的一种可行策略。本文列举了最近的一些典范进展，并讨论了蛋白融合策略的未来潜力。