Karakuzu Betul, Tarim Ergun Alperay, Oksuz Cemre, Tekin H Cumhur
Department of Bioengineering, Izmir Institute of Technology, Izmir 35430, Turkey.
METU MEMS Center, Ankara 06520, Turkey.
ACS Omega. 2022 Jul 15;7(29):25837-25843. doi: 10.1021/acsomega.2c03354. eCollection 2022 Jul 26.
Chronic kidney disease (CKD) is a high-cost disease that affects approximately one in ten people globally, progresses rapidly, results in kidney failure or dialysis, and triggers other diseases. Although clinically used serum creatinine tests are used to evaluate kidney functions, these tests are not suitable for frequent and regular control at-home settings that obstruct the regular monitoring of kidney functions, improving CKD management with early intervention. This study introduced a new electromechanical lab-on-a-chip platform for point-of-care detection of serum creatinine levels using colorimetric enzyme-linked immunosorbent assay (ELISA). The platform was composed of a chip containing microreservoirs, a stirring bar coated with creatinine-specific antibodies, and a phone to detect color generated via ELISA protocols to evaluate creatinine levels. An electromechanical system was used to move the stirring bar to different microreservoirs and stir it inside them to capture and detect serum creatinine in the sample. The presented platform allowed automated analysis of creatinine in ∼50 min down to ∼1 and ∼2 mg/dL in phosphate-buffered saline (PBS) and fetal bovine serum (FBS), respectively. Phone camera measurements in hue, saturation, value (HSV) space showed sensitive analysis compared to a benchtop spectrophotometer that could allow low-cost analysis at point-of-care.
慢性肾脏病（CKD）是一种高成本疾病，全球约十分之一的人受其影响，病情进展迅速，会导致肾衰竭或透析，并引发其他疾病。尽管临床上使用血清肌酐检测来评估肾功能，但这些检测不适用于家庭环境中的频繁和定期监测，这阻碍了肾功能的常规监测，不利于通过早期干预改善慢性肾脏病的管理。本研究引入了一种新的机电一体化芯片实验室平台，用于使用比色酶联免疫吸附测定（ELISA）在护理点检测血清肌酐水平。该平台由一个包含微储液器的芯片、一个涂有肌酐特异性抗体的搅拌棒以及一部用于检测通过ELISA方案产生的颜色以评估肌酐水平的手机组成。一个机电系统用于将搅拌棒移动到不同的微储液器中并在其中搅拌，以捕获和检测样品中的血清肌酐。所展示的平台能够在约50分钟内对肌酐进行自动分析，在磷酸盐缓冲盐水（PBS）和胎牛血清（FBS）中分别可低至约1和约2mg/dL。与台式分光光度计相比，在色调、饱和度、明度（HSV）空间中的手机摄像头测量显示出灵敏的分析效果，这使得在护理点能够进行低成本分析。
