应用超薄层等电聚焦技术对红细胞酯酶D多态性进行遗传学研究。在威尼托人群（意大利）中的分布情况。
Genetic study of red cell esterase D polymorphism by ultrathin layer isoelectric focusing. Distribution in the Veneto population (Italy).
作者信息
Cortivo P, Caenazzo L, Crestani C, Scoretti C, Benciolini P, Ongaro G
出版信息
Z Rechtsmed. 1987;98(1):39-42. doi: 10.1007/BF00200384.
Human red cell Esterase D (EsD) was analyzed by isoelectric focusing (IEF) on ultrathin-layer polyacrylamide gel with a pH range of 5.0-6.0. Hemolysates were treated with Dithiothreitol to avoid loss of activity and change of the isozyme patterns by in vitro storage effects. In our sample of 951 unrelated persons from Veneto, seven different phenotypes were observed. The following allele frequencies were calculated: EsD1 = 0.8476, EsD2 = 0.1336, EsD5 = 0.0178, and EsDV = 0.0010.
采用等电聚焦（IEF）技术，在pH范围为5.0 - 6.0的超薄层聚丙烯酰胺凝胶上对人红细胞酯酶D（EsD）进行分析。溶血产物用二硫苏糖醇处理，以避免因体外储存效应导致活性丧失和同工酶模式改变。在我们来自威尼托地区的951名无亲缘关系个体的样本中，观察到了七种不同的表型。计算出以下等位基因频率：EsD1 = 0.8476，EsD2 = 0.1336，EsD5 = 0.0178，EsDV = 0.0010。
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