Wang Lingling, Zhao Luting, Shen Lianwei, Fang Qilin, Yang Zhenglei, Wang Rongrong, Wu Qing, Xie Yulei
Department of Rehabilitation Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Department of Rehabilitation Medicine, The First People's Hospital of Ziyang, Ziyang, China.
Front Surg. 2022 Jul 15;9:911468. doi: 10.3389/fsurg.2022.911468. eCollection 2022.
Purified platelet-rich plasma (P-PRP) is gradually being used in the treatment of osteoarthritis (OA), and its sources are mainly divided into autologous and allogeneic blood. However, it is unclear whether autologous PRP is more effective or allogeneic PRP is superior.
In this study, autologous and allogeneic P-PRP was injected at early stage of KOA in rabbits, and then the differences in the efficacy of the two P-PRPs against KOA were compared from several perspectives, including pathological histology and immunohistochemistry.
Experimental rabbits were divided into normal group ( = 8), model group ( = 8), autologous P-PRP group ( = 8), and allogeneic P-PRP group ( = 8) using a random number table method. The normal and model groups did not receive any treatment, and the autologous P-PRP and allogeneic P-PRP groups received intra-articular injections of autologous and allogeneic P-PRP, respectively, to observe the changes in the gross specimens of the knee joints of the experimental rabbits in each group. The histopathological changes of chondrocytes were also observed by HE-stained sections of articular cartilage, and the expression of chondrocytes Bone morphogenetic protein-2 (BMP-2) and Sox9 were detected by immunohistochemistry.
Compared with the allogeneic P-PRP group, the differences were statistically significant ( < 0.05) in the gross specimens and pathological histological findings in the autologous PRP group. Immunohistochemical results showed that the expression of BMP-2 and Sox9 was elevated in both the autologous P-PRP group and the allogeneic P-PRP group compared with the model group, and the expression of BMP-2 was higher in the autologous P-PRP group than in the allogeneic P-PRP group, with a statistically significant difference ( < 0.05), while there was no difference in the expression of Sox9 between the two groups ( > 0.05).
Intra-articular injection of autologous P-PRP activated the expression of BMP-2 and Sox9 in chondrocytes and effectively improved KOA cartilage repair and reduced bone redundancy and joint fluid formation, and its efficacy was superior to that of intra-articular injection of allogeneic P-PRP.
纯化的富血小板血浆（P-PRP）逐渐应用于骨关节炎（OA）的治疗，其来源主要分为自体血和异体血。然而，自体PRP是否更有效或异体PRP是否更具优势尚不清楚。
本研究对兔膝骨关节炎（KOA）早期注射自体和异体P-PRP，然后从病理组织学和免疫组化等多个角度比较两种P-PRP治疗KOA的疗效差异。
采用随机数字表法将实验兔分为正常组（n = 8）、模型组（n = 8）、自体P-PRP组（n = 8）和异体P-PRP组（n = 8）。正常组和模型组不接受任何治疗，自体P-PRP组和异体P-PRP组分别接受关节腔内注射自体和异体P-PRP，观察各组实验兔膝关节大体标本的变化。通过关节软骨HE染色切片观察软骨细胞的组织病理学变化，采用免疫组化法检测软骨细胞骨形态发生蛋白-2（BMP-2）和Sox9的表达。
与异体P-PRP组相比，自体PRP组的大体标本和病理组织学结果差异有统计学意义（P < 0.05）。免疫组化结果显示，与模型组相比，自体P-PRP组和异体P-PRP组BMP-2和Sox9的表达均升高，且自体P-PRP组BMP-2的表达高于异体P-PRP组，差异有统计学意义（P < 0.05），而两组Sox9的表达差异无统计学意义（P > 0.05）。
关节腔内注射自体P-PRP可激活软骨细胞中BMP-2和Sox9的表达，有效改善KOA软骨修复，减少骨质增生和关节液形成，其疗效优于关节腔内注射异体P-PRP。
