Cakir Aysen, Ocalan Busra, Cansu Cansu, Suyen Guldal Gulec, Cansev Mehmet, Kahveci Nevzat
Department of Physiology, Bursa Uludag University School of Medicine, Bursa, Turkey.
Department of Pharmacology, Bursa Uludag University School of Medicine, Bursa, Turkey.
Iran J Basic Med Sci. 2022 May;25(5):562-568. doi: 10.22038/IJBMS.2022.60756.
Sleep has a pivotal role in learning-memory and sleep deprivation (SD) negatively affects synaptic functioning. Cytidine-5-diphosphocholine (Citicoline) has been known to improve learning and memory functions. Our objective was to explore the effects of Citicoline on hippocampal and cortical synaptic proteins in rapid eye movement (REM) sleep-deprived rats.
Rats (n=36) were randomly divided into 6 groups. Environmental control or sleep deprivation was done by placing the rat on a 13 cm diameter platform (Large Platform [LP] group) or on a 6.5 cm diameter platform (REMSD group), respectively, for 96 hours. Rats randomized for controls (Home Cage [HC] group) were followed up in home cages. Rats in each of the REMSD, LP or HC group were randomized to receive either saline (0,9%NaCl) or Citicoline (600 μmol/kg) intraperitoneally twice a day for four days. After the experiments, rats were sacrificed; their cerebral cortices and hippocampi were dissected for analyzing the levels of pre-synaptic proteins synaptophysin and synapsin I, and the post-synaptic density protein-95 (PSD-95) by Western-blotting.
Hippocampal levels of PSD-95, but not the pre-synaptic proteins, were reduced by REM sleep deprivation. Citicoline treatment ameliorated the reduction in PSD-95 levels in REM sleep-deprived rats. On the other hand, REM sleep deprivation was not found to be significantly effective on pre- or post-synaptic proteins in cerebral cortex.
REM sleep deprivation reduces hippocampal PSD-95 levels which are enhanced by Citicoline treatment. These data propose that Citicoline may ameliorate the adverse effects of SD on hippocampal synaptic functioning.
睡眠在学习记忆中起关键作用，睡眠剥夺（SD）会对突触功能产生负面影响。已知胞苷-5-二磷酸胆碱（西地那非）可改善学习和记忆功能。我们的目的是探讨西地那非对快速眼动（REM）睡眠剥夺大鼠海马和皮质突触蛋白的影响。
将36只大鼠随机分为6组。分别通过将大鼠置于直径13厘米的平台（大平台[LP]组）或直径6.5厘米的平台（REMSD组）上96小时来进行环境控制或睡眠剥夺。随机分配至对照组（家笼[HC]组）的大鼠在其家笼中进行观察。REMSD、LP或HC组中的每只大鼠随机接受生理盐水（0.9%NaCl）或西地那非（600μmol/kg）腹腔注射，每天两次，共四天。实验结束后，处死大鼠；解剖其大脑皮质和海马，通过蛋白质免疫印迹法分析突触前蛋白突触素和突触结合蛋白I以及突触后致密蛋白95（PSD-95）的水平。
REM睡眠剥夺降低了海马中PSD-95的水平，但未降低突触前蛋白的水平。西地那非治疗改善了REM睡眠剥夺大鼠中PSD-95水平的降低。另一方面，未发现REM睡眠剥夺对大脑皮质突触前或突触后蛋白有显著影响。
REM睡眠剥夺降低了海马中PSD-95的水平，而西地那非治疗可提高该水平。这些数据表明，西地那非可能改善睡眠剥夺对海马突触功能的不利影响。
