Department of Dermatology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
J Cosmet Dermatol. 2022 Nov;21(11):6343-6350. doi: 10.1111/jocd.15284. Epub 2022 Sep 2.
E-cadherin is a classic cadherin that mediates keratinocyte adhesion.
To assess the tissue expression of E-cadherin and its proteolytic serum fragment (soluble E-cadherin) in pemphigus vulgaris (PV) before and after clinical remission compared with controls.
Thirty-seven PV patients and thirty controls were enrolled. Pemphigus disease area index (PDAI) was calculated for patients at baseline and after remission. Punch biopsy specimens were taken from patients before, and after remission, and from controls for assessment of tissue E-cadherin by immunofluorescence. Similarly, serum samples were collected for assessment of serum soluble E-cadherin by ELISA.
Presence, intensity, and mean intensity of tissue E-cadherin were significantly reduced in PV patients before treatment compared with controls (p < 0.001). Detected E-cadherin showed mainly a basal and suprabasal distribution with cell surface and a cytoplasmic expression. Serum E-cadherin was significantly higher in patients before treatment compared with controls (p = 0.006). With remission, tissue E-cadherin presence, intensity, mean intensity, and serum E-cadherin showed statistically significant improvement (p = 0.003, <0.001, <0.001, and 0.003 respectively). Tissue E-cadherin presence and serum E-cadherin level reached values equivalent to the controls (p = 0.49 and 0.44, respectively).
Disruption of tissue E-cadherin and upregulation of serum soluble E-cadherin can contribute to the pathogenesis of PV. Clinical remission of PV is associated with normalization of tissue and serum E-cadherin.
E-钙黏蛋白是一种经典的钙黏蛋白，介导角质形成细胞黏附。
评估寻常型天疱疮（PV）患者在临床缓解前后与对照组相比组织中 E-钙黏蛋白及其蛋白水解血清片段（可溶性 E-钙黏蛋白）的表达。
纳入 37 例 PV 患者和 30 例对照。患者在基线和缓解后计算天疱疮疾病面积指数（PDAI）。采集患者缓解前和缓解后的皮损活检标本，通过免疫荧光法评估组织 E-钙黏蛋白。同样，采集血清样本，通过 ELISA 评估血清可溶性 E-钙黏蛋白。
与对照组相比，治疗前 PV 患者组织 E-钙黏蛋白的存在、强度和平均强度均显著降低（p<0.001）。检测到的 E-钙黏蛋白主要呈基底和超基底分布，有细胞表面和细胞质表达。治疗前患者血清 E-钙黏蛋白明显高于对照组（p=0.006）。随着缓解，组织 E-钙黏蛋白的存在、强度、平均强度和血清 E-钙黏蛋白均有统计学显著改善（p=0.003、<0.001、<0.001 和 0.003）。组织 E-钙黏蛋白的存在和血清 E-钙黏蛋白水平达到与对照组相当的水平（p=0.49 和 0.44）。
组织 E-钙黏蛋白的破坏和血清可溶性 E-钙黏蛋白的上调可能有助于 PV 的发病机制。PV 的临床缓解与组织和血清 E-钙黏蛋白的正常化相关。
