Taylor W G, Camalier R F, Tucker R W, Sanford K K
Cancer Res. 1978 Nov;38(11 Pt 1):3840-6.
The influence of population density in the progression from the nonneoplastic to the neoplastic state has been reassessed. Two twice-cloned, nonneoplastic mouse lines, NCTC 7914 and 7915, were transferred each 3 to 4 days at inoculum sizes selected to minimize or maximize cell-cell contact, 1 X 10(5) or 4 X 10(5) cells/T-15, respectively. As tested by in vivo assay, the regime designed to minimize cell-cell contact did not reproducibly delay transformation, and tumor production was observed in all lines, irrespective of inoculum size. Also, results of tumorigenesis assays correlated with blind evaluation of morphological and cytological alterations, growth in agarose, and susceptibility to killing by activated macrophages. Generally higher saturation densities were seen as a function of period in culture, and no significant differences in glucose utilization or lactic acid production were observed between nonneoplastic and neoplastic cell populations.
已重新评估了种群密度在从非肿瘤状态进展到肿瘤状态过程中的影响。选用两种经两次克隆的非肿瘤小鼠品系NCTC 7914和7915，分别以1×10⁵或4×10⁵个细胞/T - 15的接种量，每3至4天进行传代，接种量的选择旨在使细胞 - 细胞接触最小化或最大化。通过体内试验测试，旨在最小化细胞 - 细胞接触的方案并不能可重复地延迟转化，并且在所有品系中均观察到肿瘤产生，与接种量无关。此外，肿瘤发生试验的结果与对形态学和细胞学改变、在琼脂糖中的生长以及被活化巨噬细胞杀伤的敏感性的盲法评估相关。通常，随着培养时间的推移，饱和密度会更高，并且在非肿瘤细胞群体和肿瘤细胞群体之间未观察到葡萄糖利用或乳酸产生的显著差异。
