Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Division of Rheumatology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Curr Eye Res. 2022 Oct;47(10):1405-1412. doi: 10.1080/02713683.2022.2103152. Epub 2022 Aug 1.
Lat knock-in mice were recently proposed as an animal model for immunoglobulin G4 (IgG4)-related disease. In this study, we investigated whether Lat knock-in mice exhibit ophthalmic lesions, specifically in the lacrimal and Harderian glands.
Lacrimal glands, Harderian glands, and adherent lymphoid follicle lesions were dissected from Lat knock-in mice and wild type (WT) C57BL/6 mice between 6 and 24 weeks of age. Tissues were stained with hematoxylin-eosin, immunoglobulin G (IgG), and anti-IgG1, a homologue of human IgG4, for histopathological analysis.
In Lat knock-in mice, IgG1-positive cells infiltrated the space between the lacrimal gland acinar cells at 6, 9, 12, and 20 weeks or order, and the number of IgG1-positive cells did not differ significantly between these age groups. Infiltration of IgG1-positive inflammatory cell was also observed in the Harderian glands of Lat knock-in mice at all ages. The ratio of IgG1/IgG-positive cells averaged 80 and 67% in the lacrimal and Harderian glands, respectively. Dense IgG1-positive lesions were also seen in tissues adjacent to the lacrimal and Harderian glands in some Lat knock-in mice. In contrast, there were almost no IgG1-positive cell infiltrates in the lacrimal and Harderian glands of WT mice.
IgG1-positive cells infiltrate the lacrimal and Harderian glands of Lat knock-in mice, indicating that Lat knock-in mice could be a representative animal model for IgG4-related ophthalmic disease.
最近提出了 Lat 敲入小鼠作为 IgG4 相关疾病的动物模型。在这项研究中，我们研究了 Lat 敲入小鼠是否表现出眼部病变，特别是在泪腺和哈德腺。
从 6 至 24 周龄的 Lat 敲入小鼠和野生型（WT）C57BL/6 小鼠中分离出泪腺、哈德腺和附着的淋巴滤泡病变。用苏木精-伊红、免疫球蛋白 G（IgG）和抗 IgG1（人类 IgG4 的同源物）染色进行组织病理学分析。
在 Lat 敲入小鼠中，IgG1 阳性细胞在 6、9、12 和 20 周龄或顺序时渗透到泪腺腺泡细胞之间的空间，并且这些年龄组之间的 IgG1 阳性细胞数量没有显著差异。Lat 敲入小鼠的哈德腺中也观察到 IgG1 阳性炎性细胞浸润。泪腺和哈德腺中 IgG1/IgG 阳性细胞的比例分别平均为 80%和 67%。在一些 Lat 敲入小鼠的泪腺和哈德腺附近的组织中也可见到密集的 IgG1 阳性病变。相比之下，WT 小鼠的泪腺和哈德腺中几乎没有 IgG1 阳性细胞浸润。
IgG1 阳性细胞浸润 Lat 敲入小鼠的泪腺和哈德腺，表明 Lat 敲入小鼠可能是 IgG4 相关眼病的代表性动物模型。
