Institute of Chemical Sciences, Department of Interfacial Phenomena, Faculty of Chemistry, Maria Curie-Skłodowska University, M. Curie-Skłodowska Sq. 3, 20-031 Lublin, Poland; Laboratory of X-ray Optics, Department of Chemistry, Institue of Biology Sciences, Faculty of Science and Health, The John Paul II Catholic University of Lublin, Konstantynów 1J, 20-708 Lublin, Poland.
Institute of Chemical Sciences, Department of Interfacial Phenomena, Faculty of Chemistry, Maria Curie-Skłodowska University, M. Curie-Skłodowska Sq. 3, 20-031 Lublin, Poland.
Biomater Adv. 2022 Jul;138:212934. doi: 10.1016/j.bioadv.2022.212934. Epub 2022 May 29.
The main aim of the study was to determine the effect of two polysaccharides: chitosan (Ch) and hyaluronic acid (HA), and/or titanium dioxide (TiO) on the structure and behaviour of the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) membrane. To achieve this goal the surface pressure as a function of the area per molecule (π-A) isotherm for the phospholipid monolayer was recorded. The shape of the π-A isotherms and compression-decompression cycles, as well as the compression modulus values, were analysed in terms of biocompatibility. Besides, morphology and thickness of the phospholipid layers obtained by means of Brewster angle microscope at different π, were determined. The obtained results show that both polysaccharides Ch, HA, as well inorganic TiO affect slightly the structure of the DOPC monolayer but do not disrupt it. Their presence brings no typical arrangements of both the polar heads and tails of DOPC molecules at the interface.
本研究的主要目的是确定两种多糖（壳聚糖（Ch）和透明质酸（HA））和/或二氧化钛（TiO）对 1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）膜的结构和行为的影响。为了实现这一目标，记录了磷脂单层的表面压力作为每个分子面积（π-A）等温线的函数。根据生物相容性分析了π-A 等温线和压缩-减压循环以及压缩模量值的形状，此外，还通过布鲁斯特角显微镜在不同π下确定了磷脂层的形态和厚度。得到的结果表明，两种多糖 Ch 和 HA 以及无机 TiO 都对 DOPC 单层的结构有轻微影响，但不会破坏它。它们的存在不会在界面处产生 DOPC 分子的极性头和尾部的典型排列。
