Singha Shubham, Pandey Mamta, Jaiswal Latika, Dash Sangram, Fernandes Abhijeet, Kumaresan Arumugan, Maharana Biswa Ranjan, Lathwal Surender Singh, Sarath Thulasiraman, Datta Tirtha K, Mohanty Tushar K, Baithalu Rubina Kumari
Animal Reproduction, Gynaecology and Obstetrics, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Molecular Reproduction Lab, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
Anim Biotechnol. 2023 Dec;34(7):2554-2564. doi: 10.1080/10495398.2022.2105228. Epub 2022 Aug 1.
Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarkers; therefore, the present study was carried out to evaluate their potential as estrus biomarkers. The levels of , , , transcripts were compared in saliva during estrous cycle stages [early proestrus (day -2, EP), late proestrus (day-1, LP), estrus (E), metestrus (ME) and diestrus (DE)] of cyclic heifers ( = 8) and pluriparous ( = 8) buffaloes by employing quantitative real-time polymerase chain reaction (qRT-PCR). The levels of (EP/DE 1.46-2.43 fold, LP/DE 2.49-3.06 fold; E/DE 7.21-11.9-fold < 0.01; ME/D 1.0-1.16 fold) and (EP/DE 0.93-2.39 fold, LP/DE 2.68-3.23 fold; E/DE 8.52-15.18 fold < 0.01; ME/D 0.86-1.01 fold) were significantly altered during the estrus than other estrous cycle stages in both cyclic heifers and pluriparous buffaloes. Receiver operating characteristic curve analysis revealed the ability of salivary (AUC 0.96; < 0.001) and (AUC 0.99; < 0.01) to differentiate E from other stages of the estrous cycle. Significantly higher levels of and transcripts in saliva during the estrus phase suggest their biomarkers potential for estrus detection in buffaloes.
由于发情迹象表现不佳导致繁殖效率低下，发情检测是水牛养殖中的一个主要问题。唾液转录本分析是一种很有前景的识别生物标志物的工具；因此，本研究旨在评估其作为发情生物标志物的潜力。通过定量实时聚合酶链反应（qRT-PCR）比较了周期性小母牛（n = 8）和经产（n = 8）水牛在发情周期各阶段[发情前期早期（第-2天，EP）、发情前期晚期（第-1天，LP）、发情期（E）、发情后期（ME）和间情期（DE）]唾液中 、 、 、 转录本的水平。在周期性小母牛和经产水牛中，与其他发情周期阶段相比，发情期时 （EP/DE为1.46 - 2.43倍，LP/DE为2.49 - 3.06倍；E/DE为7.21 - 11.9倍，P < 0.01；ME/D为1.0 - 1.16倍）和 （EP/DE为0.93 - 2.39倍，LP/DE为2.68 - 3.23倍；E/DE为8.52 - 15.18倍，P < 0.01；ME/D为0.86 - 1.01倍）的水平均有显著变化。受试者工作特征曲线分析显示，唾液 （AUC为0.96；P < 0.001）和 （AUC为0.99；P < 0.01）能够区分发情期与发情周期的其他阶段。发情期唾液中 和 转录本水平显著更高，表明它们在水牛发情检测中具有作为生物标志物的潜力。
