从角膜脓肿中分离出的莫拉菌属菌株的遗传多样性研究。
Study of the genetic diversity of Moraxella spp. isolates obtained from corneal abscesses.
作者信息
Roitman Karina L, Cipolla Lucía, Minervini Patricia, Prieto Mónica
机构信息
Residencia de Microbiología Clínica, Instituto Nacional de Enfermedades Infecciosas (INEI), Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina.
Servicio de Bacteriología Especial, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas (INEI), Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) «Dr. Carlos G. Malbrán», Ciudad Autónoma de Buenos Aires, Argentina.
出版信息
Rev Argent Microbiol. 2023 Jan-Mar;55(1):20-24. doi: 10.1016/j.ram.2022.03.004. Epub 2022 Jul 29.
This is the first study of the genetic diversity of Moraxella spp. Isolates were detected in an Eye Hospital in the City of Buenos Aires. Due to the high frequency of Moraxella spp. observed in corneal abscesses, we decided to validate their identification at the species level, determine their drug susceptibility and perform molecular subtyping. Seventeen (17) isolates obtained from corneal abscesses were evaluated. The identification was carried out using a combination of biochemical tests and MALDI-TOF mass spectrometry. Of these isolates, 88.2% were identified as Moraxella lacunata, and 11.8% as Moraxella nonliquefaciens. Molecular subtyping was performed using the pulsed-field gel electrophoresis (PFGE) technique. All isolates were typable and thirteen digestion patterns were identified. Based on the obtained results, the PFGE technique using the SmaI enzyme can be used for epidemiological studies of strains of these species.
这是关于莫拉克斯氏菌属遗传多样性的首次研究。在布宜诺斯艾利斯市的一家眼科医院检测到了分离株。由于在角膜脓肿中观察到莫拉克斯氏菌属的高频率，我们决定在种水平上验证其鉴定结果，确定其药敏性并进行分子分型。对从角膜脓肿中获得的17株分离株进行了评估。使用生化试验和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）相结合的方法进行鉴定。在这些分离株中，88.2%被鉴定为腔隙莫拉克斯氏菌，11.8%为非液化莫拉克斯氏菌。使用脉冲场凝胶电泳（PFGE）技术进行分子分型。所有分离株均可分型，共鉴定出13种酶切图谱。基于获得的结果，使用SmaI酶的PFGE技术可用于这些菌种的菌株的流行病学研究。
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