LaCroce Samantha J, Wilson Mollie N, Romanowski John E, Newman Jeffrey D, Jhanji Vishal, Shanks Robert M Q, Kowalski Regis P
Department of Ophthalmology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Clinical Laboratory-Microbiology, University of Pittsburgh Medical Center, Pittsburgh, PA 16148, USA.
Microorganisms. 2019 Jun 4;7(6):163. doi: 10.3390/microorganisms7060163.
is an ocular bacterial pathogen isolated in cases of keratitis, conjunctivitis, and endophthalmitis. Gram-negative brick-shaped diplobacilli from ocular specimens, and slow growth in culture, are early indications of ocular infection; however, identifying to species can be complex and inconsistent. In this study, bacteria consistent with were identified to species using: (1) DNA sequencing coupled with vancomycin susceptibility, (2) MALDI-TOF mass spectrometry, and (3) the Biolog ID system. Study samples consisted of nine ATCC controls, 82 isolates from keratitis, 21 isolates from conjunctivitis, and 4 isolates from endophthalmitis. The ATCC controls were correctly identified. For keratitis, 66 (80.5%) were identified as , 7 (9.0%) as , 5 (6%) as , 2 (2.5%) as , 1 (1.0%) as , and 1 (1.0%) as . For conjunctivitis, 9 (43.0%) were identified as , 6 (29.0%) as , 3 (14.3%) as , 2 (9.5%) as (), and 1 (4.5%) as . From endophthalmitis, 3 of 4 of the isolates were . Overall, . and were identified in 70% (75 of 107) and 13% (14 of 107) of cases, respectively, totaling 83% (89 of 107). and are important bacterial pathogens of the eye as determined by DNA sequencing, MALDI-TOF MS, and Biolog. Although is a clinical pathogen, other species of appear to have a prominent role in eye infections.
是一种在角膜炎、结膜炎和眼内炎病例中分离出的眼部细菌病原体。眼部标本中的革兰氏阴性砖形双杆菌以及培养中生长缓慢是眼部感染的早期迹象；然而，鉴定到种可能很复杂且不一致。在本研究中，使用以下方法将与该菌相符的细菌鉴定到种：(1) 结合万古霉素敏感性的DNA测序，(2) 基质辅助激光解吸电离飞行时间质谱，以及(3) Biolog ID系统。研究样本包括9个美国典型培养物保藏中心（ATCC）对照、82株来自角膜炎的分离株、21株来自结膜炎的分离株和4株来自眼内炎的分离株。ATCC对照被正确鉴定。对于角膜炎，66株（80.5%）被鉴定为该菌，7株（9.0%）为另一菌，5株（6%）为又一菌，2株（2.5%）为某菌，1株（1.0%）为另一菌，1株（1.0%）为又一菌。对于结膜炎，9株（43.0%）被鉴定为该菌，6株（29.0%）为另一菌，3株（14.3%）为又一菌，2株（9.5%）为某菌（括号内为另一菌名），1株（4.5%）为另一菌。来自眼内炎的4株分离株中有3株为该菌。总体而言，该菌和另一菌分别在70%（107例中的75例）和13%（107例中的14例）的病例中被鉴定到，总计83%（107例中的89例）。通过DNA测序、基质辅助激光解吸电离飞行时间质谱和Biolog确定，该菌和另一菌是眼部重要的细菌病原体。虽然该菌是一种临床病原体，但该菌的其他种似乎在眼部感染中起重要作用。
