miR-150 和 miR-21 表达水平对弥漫性大 B 细胞淋巴瘤的生化和临床影响。

Biochemical and clinical impacts of miR-150 and miR-21 expression levels in diffuse large B cell lymphoma.

机构信息

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine - Menoufia University, Shibin Al Kawm, Egypt.

Department of chemistry, Faculty of science-Menoufia University, Shibin Al Kawm, Egypt.

出版信息

J Immunoassay Immunochem. 2022 Nov 2;43(6):648-664. doi: 10.1080/15321819.2022.2103431. Epub 2022 Aug 1.

DOI:10.1080/15321819.2022.2103431

PMID:35915976

Abstract

Identification of biomarkers is crucial in guiding the treatment decision and improving the future outcomes of DLBCL. The aim of the current study is to detect the biochemical and clinical impacts of miR-150 and miR-21 expression levels in DLBCL. Quantification of serum miR-150 and miR-21 expression levels by real-time PCR after micro-RNA extraction and RT-PCR. At a cutoff point of 2.3 for miR-21, the sensitivity, specificity, positive predictive, and negative predictive values for diagnosis of DLBCL were 98%, 90%, 90.7%, and 97.8%, respectively. At cut-off point (≤19.12) the sensitivity, specificity, the positive predictive and negative predictive values of miR-21 to discriminate stage IV vs stage II DLBCL patients were 68.42%, 80%, 86.7%%,and 57.1%, respectively. Serum miR-150 and serum miR-21 can be used as diagnostic markers for DLBCL patients, but miR-21 is more sensitive than miR-150. Serum miR-21 can be used as prognostic marker for DLBCL patients. It was more sensitive and more specific than miR-150.

摘要

鉴定生物标志物对于指导治疗决策和改善弥漫性大 B 细胞淋巴瘤（DLBCL）的未来结局至关重要。本研究旨在检测 miR-150 和 miR-21 表达水平对 DLBCL 的生化和临床影响。通过 micro-RNA 提取和 RT-PCR 后实时 PCR 定量血清 miR-150 和 miR-21 表达水平。miR-21 的截断值为 2.3 时，用于诊断 DLBCL 的敏感度、特异度、阳性预测值和阴性预测值分别为 98%、90%、90.7%和 97.8%。在截断值（≤19.12）时，miR-21 区分 IV 期和 II 期 DLBCL 患者的敏感度、特异度、阳性预测值和阴性预测值分别为 68.42%、80%、86.7%和 57.1%。血清 miR-150 和血清 miR-21 可作为 DLBCL 患者的诊断标志物，但 miR-21 比 miR-150 更敏感。血清 miR-21 可作为 DLBCL 患者的预后标志物，其敏感度和特异度均高于 miR-150。