Protegenomics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute, Southern Regional Station, Bengaluru, India.
Reprod Domest Anim. 2022 Nov;57(11):1450-1464. doi: 10.1111/rda.14222. Epub 2022 Aug 16.
Anti-Müllerian hormone (AMH) is a member of the TGF-β superfamily produced by follicular granulosa cells in women and cattle and is considered an endocrine biomarker of ovarian follicular reserve. The study examined how age and parity influence serum AMH concentration and investigated the presence of single nucleotide polymorphisms in AMH gene in Bos indicus breeds viz Malnad Gidda Amritmahal and Hallikar. All five exons of AMH gene amplified by polymerase chain reaction were subjected to sanger sequencing and identified important SNP and its effects. We observed a highly significant relationship between parity and AMH concentration in Amritmahal cattle, whereas Malnad Gidda and Hallikar breeds did not show a significant difference. We identified one SNP located in exon 5 (rs21402788) with base change A>G, a non-synonymous mutation resulting in a change in amino acid Q>R and the protein product. It is concluded that AMH level could be considered as an indicator of the ovarian reserve and productive herd life (longevity) irrespective of age/parity, especially in B. indicus breeds of cattle.
抗缪勒管激素（AMH）是转化生长因子-β超家族的成员，由女性和牛的卵泡颗粒细胞产生，被认为是卵巢卵泡储备的内分泌生物标志物。本研究探讨了年龄和产次如何影响血清 AMH 浓度，并研究了 Bos indicus 品种（如 Malnad Gidda Amritmahal 和 Hallikar）中 AMH 基因的单核苷酸多态性的存在。通过聚合酶链反应扩增 AMH 基因的所有五个外显子，并进行 Sanger 测序，以鉴定重要的 SNP 及其影响。我们观察到 Amritmahal 牛的产次与 AMH 浓度之间存在高度显著的关系，而 Malnad Gidda 和 Hallikar 品种则没有显著差异。我们在 5 号外显子（rs21402788）中鉴定到一个 SNP，其碱基变化为 A>G，导致氨基酸 Q>R 的非同义突变和蛋白质产物的变化。结论是，AMH 水平可以被认为是卵巢储备和生产牛群寿命（长寿）的指标，与年龄/产次无关，特别是在 Bos indicus 牛品种中。
