Ambreetha Sakthivel, Balachandar Dananjeyan
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
J Basic Microbiol. 2023 Jan;63(1):4-16. doi: 10.1002/jobm.202200419. Epub 2022 Aug 2.
Microbial inoculants are globally recommended for plant growth promotion and control of plant pathogens. These inoculants require stringent quality checks for sustainable field efficacy. Questionable regulatory frameworks constantly deteriorate the reliability of bio-inoculant technology. Existing global regulations do not involve any rapid molecular technique for the routine inspection of microbial preparations. Sequence characterized amplified region (SCAR) marker offers rapid and precise strain-level authentication of target microbes. Such advanced molecular techniques must be exploited to accurately validate the microbial formulations. Besides, the global dissemination of plant pathogenic microbes has always been an alarming threat to food security. SCAR markers could be used at the plant quarantine centers to rapidly detect catastrophic pathogens, thereby circumventing the import and export of contagious plant materials. The current review is focused on promoting the SCAR marker technology to validate commercial bio-inoculants and predict plant pandemics.
微生物接种剂在全球范围内被推荐用于促进植物生长和控制植物病原体。这些接种剂需要进行严格的质量检查以确保田间可持续有效性。有问题的监管框架不断降低生物接种剂技术的可靠性。现有的全球法规未涉及用于微生物制剂常规检查的任何快速分子技术。序列特征扩增区域(SCAR)标记提供了对目标微生物的快速且精确的菌株水平鉴定。必须利用这种先进的分子技术来准确验证微生物制剂。此外,植物病原微生物的全球传播一直是对粮食安全的一个令人担忧的威胁。SCAR标记可用于植物检疫中心以快速检测灾难性病原体,从而避免传染性植物材料的进出口。当前的综述聚焦于推广SCAR标记技术以验证商业生物接种剂并预测植物大流行。