Li Chong, Jia Zhaohui, Ma Shilin, Liu Xin, Zhang Jinchi, Müller Christoph
Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.
Institute of Plant Ecology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26, 35392 Giessen, Germany.
Microorganisms. 2023 Feb 24;11(3):570. doi: 10.3390/microorganisms11030570.
Microbial inoculants can be used to restore abandoned mines because of their positive effects on plant growth and soil nutrients. Currently, soils in greenhouse pot studies are routinely sterilized to eradicate microorganisms, allowing for better inoculant colonization. Large-scale field sterilization of abandoned mining site soils for restoration is difficult, though. In addition, microbial inoculants have an impact on plants. Plants also have an impact on local microbes. The interactions among microbial inoculants, native microorganisms, and plants, however, have not been studied. We created a pot experiment utilizing the soil and microbial inoculant from a previous experiment because it promoted plant growth in that experiment. To evaluate the effects of the plants, native microorganisms, and microbial inoculants, we assessed several indicators related to soil elemental cycling and integrated them into the soil multifunctionality index. The addition of the microbial inoculant and sterilizing treatment had a significant impact on alfalfa growth. When exposed to microbial inoculant treatments, the plant and sterilization treatments displayed radically different functional characteristics, where most of the unsterilized plant treatment indices were higher than those of the others. The addition of microbial inoculant significantly increased soil multifunctionality in plant treatments, particularly in the unsterilized plant treatment, where the increase in soil multifunctionality was 260%. The effect size result shows that the positive effect of microbial inoculant on soil multifunctionality and unsterilized plant treatment had the most significant promotion effect. Plant and native microorganisms amplify the positive effects of microbial inoculant.
微生物接种剂因其对植物生长和土壤养分有积极影响，可用于恢复废弃矿山。目前，温室盆栽研究中的土壤通常会进行灭菌以根除微生物，从而使接种剂更好地定殖。然而，对废弃矿场土壤进行大规模田间灭菌以进行恢复是困难的。此外，微生物接种剂对植物有影响。植物也会对当地微生物产生影响。然而，微生物接种剂、本地微生物和植物之间的相互作用尚未得到研究。我们利用之前实验中的土壤和微生物接种剂进行了盆栽实验，因为在该实验中它促进了植物生长。为了评估植物、本地微生物和微生物接种剂的影响，我们评估了与土壤元素循环相关的几个指标，并将它们整合到土壤多功能性指数中。添加微生物接种剂和灭菌处理对苜蓿生长有显著影响。当接受微生物接种剂处理时，植物和灭菌处理表现出截然不同的功能特征，其中大多数未灭菌的植物处理指标高于其他处理。添加微生物接种剂显著提高了植物处理中的土壤多功能性，特别是在未灭菌的植物处理中，土壤多功能性增加了260%。效应大小结果表明，微生物接种剂对土壤多功能性的积极影响以及对未灭菌植物处理的促进作用最为显著。植物和本地微生物放大了微生物接种剂的积极影响。
