Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Oral Disease, Sichuan University, Chengdu, China.
Eur J Oral Sci. 2022 Oct;130(5):e12887. doi: 10.1111/eos.12887. Epub 2022 Aug 2.
The purpose of this study was to develop a chewing gum containing a novel antimicrobial peptide GH12 and evaluate its biocompatibility, antimicrobial activity, and caries-preventive effects in vivo and in vitro. GH12 chewing gum was developed using a conventional method and its extracts were prepared in artificial saliva. GH12 concentration in the extracts was determined by high-performance liquid chromatography; extracts were used for growth curve assay, time-kill assay, crystal violet staining assay, scanning electron microscopy, and Cell Counting Kit-8 assay. A rat caries model was established, and molars were treated topically with extracts for 5 weeks. Weight gain monitoring, hematoxylin-eosin staining, micro-computed tomography, and Keyes scoring were conducted. Significant inhibition of Streptococcus mutans growth and biofilm formation was observed. Extracts displayed low cytotoxicity against human gingival epithelial cells. No significant differences in weight gain or signs of harm to the mucosal tissues in any of the rats were observed. Keyes scores of caries lesions in the GH12 chewing gum group were lower than those of the negative control group. It was concluded that GH12 chewing gum showed good biocompatibility, antimicrobial activity, and caries-preventive effects, exhibiting great potential to prevent dental caries as an adjuvant to regular oral hygiene.
本研究旨在开发一种含有新型抗菌肽 GH12 的口香糖，并评估其在体内和体外的生物相容性、抗菌活性和防龋效果。采用常规方法制备 GH12 口香糖，在人工唾液中提取其提取物。采用高效液相色谱法测定提取物中 GH12 的浓度；采用生长曲线法、杀菌试验、结晶紫染色法、扫描电子显微镜和细胞计数试剂盒-8 法对提取物进行检测。建立大鼠龋病模型，将提取物局部涂于磨牙上 5 周。进行体重监测、苏木精-伊红染色、微计算机断层扫描和 Keyes 评分。观察到对变形链球菌生长和生物膜形成的显著抑制作用。提取物对人牙龈上皮细胞的细胞毒性较低。未观察到任何大鼠体重增加或粘膜组织损伤的迹象。GH12 口香糖组龋病病变的 Keyes 评分低于阴性对照组。综上所述，GH12 口香糖具有良好的生物相容性、抗菌活性和防龋效果，有望作为常规口腔卫生的辅助手段预防龋齿。
