Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Patologia Geral, Belo Horizonte, MG, Brasil.
Mem Inst Oswaldo Cruz. 2022 Aug 1;117:e220065. doi: 10.1590/0074-02760220065. eCollection 2022.
Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle.
Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host.
Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism - PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene.
Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations.
Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus.
曼氏利什曼原虫（Mundinia）是豚鼠（Cavia porcellus）中常见的一种物种。尽管它是一种嗜皮物种，但对于其在利什曼生命周期中是否能够内脏化仍然存在不确定性。
本研究旨在调查曼氏利什曼原虫（L88 株）在其自然宿主豚鼠的肺部、气管、脾脏和肝脏中内脏化的能力。
动物通过鼻内皮下感染，在 12 周内使用组织学（苏木精-伊红）和分子工具（聚合酶链反应-限制性片段长度多态性 - PCR-RFLP）进行监测。为了从豚鼠中分离寄生虫，对动物进行了实验性感染，以便在进行内脏切除和针对 hsp70 基因的 PCR 分型时分离寄生虫。
组织学分析显示，在感染后 4 至 8 周，气管、肺和脾脏中存在强烈而弥漫的炎症，伴有阿米巴原虫。分子分析石蜡包埋组织在气管和脾脏中检测到了寄生虫 DNA。在 12 周 PI 时，未在任何器官中检测到寄生虫 DNA。为了确认脾脏可以作为曼氏利什曼原虫的临时栖息地，我们进行了额外的体内实验。在 6 周 PI 时，从脾脏中分离出了寄生虫，证实了先前的组织病理学和 PCR 观察结果。
曼氏利什曼原虫（L88 株）能够在豚鼠的气管、肺和脾脏中内脏化。
