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从皮肤趋向性的婴儿利什曼原虫中提取的脂磷葡聚糖比内脏趋向性菌株的更具炎症性。

Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains.

机构信息

Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Belo Horizonte, MG, Brasil.

Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia de Moléstias Infecciosas, São Paulo, SP, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2020 Sep 21;115:e200140. doi: 10.1590/0074-02760200140. eCollection 2020.

DOI:10.1590/0074-02760200140
PMID:32965329
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7521155/
Abstract

Although Leishmania infantum is well-known as the aethiological agent of visceral leishmaniasis (VL), in some Central American countries it may cause atypical non-ulcerated cutaneous leishmaniasis (NUCL). However, the mechanisms favoring its establishment in the skin are still unknown. Lipophosphoglycan (LPG) is the major Leishmania multivirulence factor involved in parasite-host interaction. In the case of viscerotropic L. infantum, it causes an immunosuppression during the interaction with macrophages. Here, we investigated the biochemical and functional roles of LPGs from four dermotropic L. infantum strains from Honduras during in vitro interaction with murine macrophages. LPGs were extracted, purified and their repeat units analysed. They did not have side chains consisting of Gal(β1,4)Man(α1)-PO4 common to all LPGs. Peritoneal macrophages from BALB/c and C57BL/6 were exposed to LPG for nitric oxide (NO) and cytokine (TNF-α and, IL-6) production. LPGs from dermotropic strains from Honduras triggered higher NO and cytokine levels compared to those from viscerotropic strains. In conclusion, LPGs from dermotropic strains are devoid of side-chains and exhibit high pro-inflammatory activity.

摘要

虽然利什曼原虫是内脏利什曼病（VL）的已知病原体，但在一些中美洲国家，它可能导致非溃疡性皮肤利什曼病（NUCL）。然而，有利于其在皮肤中建立的机制尚不清楚。脂磷壁酸（LPG）是参与寄生虫与宿主相互作用的主要利什曼多毒力因子。在亲内脏的利什曼原虫的情况下，它在与巨噬细胞相互作用时引起免疫抑制。在这里，我们研究了来自洪都拉斯的四种亲皮利什曼原虫株与小鼠巨噬细胞体外相互作用时的 LPG 的生化和功能作用。提取、纯化了 LPG 并分析了它们的重复单位。它们没有侧链，这些侧链由所有 LPG 共有的 Gal(β1,4)Man(α1)-PO4 组成。BALB/c 和 C57BL/6 的腹腔巨噬细胞暴露于 LPG 以产生一氧化氮（NO）和细胞因子（TNF-α 和 IL-6）。与亲内脏菌株相比，来自洪都拉斯亲皮菌株的 LPG 触发了更高水平的 NO 和细胞因子。总之，亲皮菌株的 LPG 没有侧链，表现出很高的促炎活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/de81a603f931/1678-8060-mioc-115-e200140-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/2955948cd18a/1678-8060-mioc-115-e200140-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/de91d7cd8135/1678-8060-mioc-115-e200140-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/de81a603f931/1678-8060-mioc-115-e200140-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/2955948cd18a/1678-8060-mioc-115-e200140-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/de91d7cd8135/1678-8060-mioc-115-e200140-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c82/7521155/de81a603f931/1678-8060-mioc-115-e200140-gf3.jpg

相似文献

[1]
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[2]
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[3]
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[4]
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[5]
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[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

[1]
A comprehensive phenotypic and genotypic taxonomic review of Leishmania (Leishmania) poncei n. sp. (Kinetoplastea: Trypanosomatidae): a novel agent of cutaneous (non-ulcerated) and visceral leishmaniasis in Honduras, Central America.

Trop Dis Travel Med Vaccines. 2025-7-28

[2]
Infectivity studies of Leishmania (Leishmania) infantum chagasi isolated from non-ulcerated cutaneous leishmaniasis.

Rev Inst Med Trop Sao Paulo. 2025-4-4

[3]
Activation Pathways of Murine Macrophages by Lipophosphoglycan from Strains of (FV1 and LV39).

ACS Infect Dis. 2024-10-11

[4]
Unveiling the Enigmatic nature of six neglected Amazonian Leishmania (Viannia) species using the hamster model: Virulence, Histopathology and prospection of LRV1.

PLoS Negl Trop Dis. 2024-8

[5]
Isolation and characterisation of Leishmania (Leishmania) infantum from cutaneous leishmaniasis patients in northeast Brazil.

Mem Inst Oswaldo Cruz. 2024

[6]
Transmission-Blocking Vaccines for Canine Visceral Leishmaniasis: New Progress and Yet New Challenges.

Vaccines (Basel). 2023-10-5

[7]
Phenotypical Differences between () PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies.

Pathogens. 2023-1-22

[8]
Leishmania amazonensis from distinct clinical forms/hosts has polymorphisms in Lipophosphoglycans, displays variations in immunomodulatory properties and, susceptibility to antileishmanial drugs.

Cell Biol Int. 2022-11

[9]
Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus.

Mem Inst Oswaldo Cruz. 2022

[10]
Lipophosphoglycan From Dermotropic New World Upregulates Interleukin-32 and Proinflammatory Cytokines Through TLR4 and NOD2 Receptors.

Front Cell Infect Microbiol. 2022

本文引用的文献

[1]
Intraspecies Polymorphisms in the Lipophosphoglycan of Differentially Modulate Macrophage Activation via TLR4.

Front Cell Infect Microbiol. 2019-7-10

[2]
Histopathological features of skin lesions in patients affected by non-ulcerated or atypical cutaneous leishmaniasis in Honduras, Central America.

Int J Exp Pathol. 2018-11-27

[3]
Salivary Gland Extract Modulates the Infection of Two Strains by Interfering With Macrophage Differentiation in the Model of .

Front Microbiol. 2018-5-29

[4]
Evaluation of Regulatory Immune Response in Skin Lesions of Patients Affected by Nonulcerated or Atypical Cutaneous Leishmaniasis in Honduras, Central America.

Mediators Inflamm. 2018-3-21

[5]
Gut Microbes Egested during Bites of Infected Sand Flies Augment Severity of Leishmaniasis via Inflammasome-Derived IL-1β.

Cell Host Microbe. 2017-12-28

[6]
Leishmania infantum lipophosphoglycan induced-Prostaglandin E production in association with PPAR-γ expression via activation of Toll like receptors-1 and 2.

Sci Rep. 2017-10-30

[7]
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.

Parasitol Int. 2017-2

[8]
Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection.

PLoS Negl Trop Dis. 2016-8-10

[9]
Differential modulation of macrophage response elicited by glycoinositolphospholipids and lipophosphoglycan from Leishmania (Viannia) shawi.

Parasitol Int. 2015-8

[10]
Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.

Parasit Vectors. 2015-1-17

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