Department of Microbiology, University of Illinois at Urbana-Champaigngrid.35403.31, Urbana, Illinois, USA.
Department of Biochemistry, University of Illinois at Urbana-Champaigngrid.35403.31, Urbana, Illinois, USA.
J Bacteriol. 2022 Sep 20;204(9):e0020222. doi: 10.1128/jb.00202-22. Epub 2022 Aug 3.
Enterococcus faecalis encodes two proteins, AcpA and AcpB, having the characteristics of acyl carrier proteins (ACPs). We report that the gene located in the fatty acid synthesis operon is essential for fatty acid synthesis and the Δ strain requires unsaturated fatty acids for growth. The Δ strain could be complemented by a plasmid carrying a wild-type gene, but not by a plasmid carrying a wild-type gene. Substitution of four AcpA residues for those of AcpB resulted in a protein that modestly complemented the Δ strain and restored fatty acid synthesis, although the acyl chains synthesized were unusually short. Enterococcus faecalis, as well as related species, has two genes- and -encoding putative acyl carrier proteins (ACPs). It has been assumed that AcpA is essential for fatty acid synthesis whereas AcpB is involved utilization of environmental fatty acids. We report here the first experimental test of the essentiality of and show that it is indeed an essential gene that cannot be replaced by .
屎肠球菌编码两种具有酰基载体蛋白 (ACP) 特征的蛋白，AcpA 和 AcpB。我们报告说，位于脂肪酸合成操纵子中的 基因对于脂肪酸合成是必需的，Δ 株需要不饱和脂肪酸才能生长。野生型 基因的质粒可互补 Δ 株，但野生型 基因的质粒不可互补。将 AcpA 的四个残基替换为 AcpB 的残基导致产生一种蛋白，该蛋白适度地互补了 Δ 株并恢复了脂肪酸合成，尽管合成的酰基链非常短。屎肠球菌以及相关物种有两个基因-和-编码假定的酰基载体蛋白 (ACP)。人们一直认为 AcpA 对于脂肪酸合成是必需的，而 AcpB 则参与环境脂肪酸的利用。我们在这里首次对 的必需性进行了实验测试，并表明它确实是一个必需基因，不能被 取代。
