Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Analyst. 2022 Aug 22;147(17):3833-3837. doi: 10.1039/d2an01048h.
Using the DNA activator of Cas to label the secondary antibody of traditional ELISA, the CRISPR/Cas12a-based fluorescence immunoassay was presented. The sensing strategy ingeniously combines the efficient signal generation of the CRISPR/Cas12a system with antigen-antibody-specific recognition. This work provides an alternative strategy to expand the application of the CRISPR/Cas system for the detection of non-nucleic acid targets.
采用 Cas 的 DNA 激活剂对传统 ELISA 的二抗进行标记，构建了基于 CRISPR/Cas12a 的荧光免疫分析方法。该传感策略巧妙地结合了 CRISPR/Cas12a 系统的高效信号产生与抗原-抗体特异性识别。这项工作为扩展 CRISPR/Cas 系统在非核酸靶标检测中的应用提供了一种替代策略。
