Department of Critical Care Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
Department of Burn Orthopaedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
Immunol Invest. 2022 Oct;51(7):2086-2096. doi: 10.1080/08820139.2022.2107932. Epub 2022 Aug 3.
Cardiac dysfunction is the most common clinical complication of sepsis. Herein, the study explored the clinical importance of long non-coding RNA (lncRNA) HOXA terminal transcript antisense RNA (HOTTIP) in the onset of sepsis and the development of cardiac dysfunction.
120 patients with sepsis were recruited and divided into cardiac dysfunction group and non-cardiac dysfunction group. Serum HOTTIP levels were measured via RT-qPCR. AC16 cells were treated with lipopolysaccharide (LPS) for cell experiments and detected for cell viability and apoptosis.
High serum HOTTIP levels were tested in sepsis patients, which was associated with procalcitonin (PCT) level. Serum HOTTIP can identify sepsis cases from healthy people with the AUC of 0.927. 72 cases developed into cardiac dysfunction, accompanied by elevated levels of HOTTIP. ROC curve displayed the predictive ability of serum HOTTIP in the development of cardiac dysfunction in patients with sepsis. After adjusting for other clinical parameters, HOTTIP can independently affect the development of cardiac dysfunction. In vitro, HOTTIP knockdown promoted the recovery of cell viability and reversed LPS-induced cell apoptosis and excessive interleukin-6 (IL-6) release.
LncRNA HOTTIP is closely related to the condition of patients with sepsis and the development of cardiac dysfunction, possibly owing to its function in LPS-induced myocardial apoptosis and inflammation.
心功能障碍是脓毒症最常见的临床并发症。本研究探讨了长链非编码 RNA（lncRNA）HOXA 末端转录反义 RNA（HOTTIP）在脓毒症发病和心功能障碍发展中的临床意义。
招募了 120 例脓毒症患者，并将其分为心功能障碍组和非心功能障碍组。通过 RT-qPCR 测量血清 HOTTIP 水平。用脂多糖（LPS）处理 AC16 细胞进行细胞实验，并检测细胞活力和细胞凋亡。
脓毒症患者的血清 HOTTIP 水平较高，与降钙素原（PCT）水平相关。血清 HOTTIP 可以从健康人群中识别出脓毒症患者，AUC 为 0.927。72 例患者发展为心功能障碍，伴有 HOTTIP 水平升高。ROC 曲线显示血清 HOTTIP 对脓毒症患者心功能障碍发展的预测能力。在调整其他临床参数后，HOTTIP 可以独立影响心功能障碍的发展。在体外，HOTTIP 敲低可促进细胞活力的恢复，并逆转 LPS 诱导的细胞凋亡和过度白细胞介素 6（IL-6）释放。
lncRNA HOTTIP 与脓毒症患者的病情和心功能障碍的发展密切相关，可能与其在 LPS 诱导的心肌细胞凋亡和炎症中的作用有关。
