School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
Plant Foods Hum Nutr. 2022 Sep;77(3):455-459. doi: 10.1007/s11130-022-00999-8. Epub 2022 Aug 3.
Coriandrum sativum L. (coriander), which is an annual herb of the Apiaceae family, has been traditionally used as a remedy. Here we tested whether heated extract of coriander leaf protects nigral dopaminergic neurodegeneration after exposure to 6-hydroxydopamine (6-OHDA). After injection of 6-OHDA into the rat substantia nigra pars compacta (SNpc), dopaminergic degeneration, which was determined by tyrosine hydroxylase immunostaining, was rescued by co-injection of CaEDTA, an extracellular Zn chelator, suggesting that extracellular Zn influx is involved in neurodegeneration. Both intracellular Zn dysregulation determined by ZnAF-2 fluorescence and dopaminergic degeneration in the SNpc induced by 6-OHDA were rescued by co-injection of 0.25% coriander extract, which also reduced reactive oxygen species (ROS) production in the SNpc determined by aminophenyl fluorescein fluorescence. The present study suggests that coriander leaf extract protects nigral dopaminergic neurodegeneration induced by intracellular Zn dysregulation. It is likely that the nutraceutical property of coriander leaf extract contributes to the protection via reducing ROS production.
芫荽（芫荽），一种伞形科一年生草本植物，已被传统用作药物。在这里，我们测试了芫荽叶的加热提取物是否能保护暴露于 6-羟多巴胺（6-OHDA）后黑质多巴胺能神经退行性变。6-OHDA 注入大鼠黑质致密部（SNpc）后，酪氨酸羟化酶免疫染色确定多巴胺能变性通过共注射 CaEDTA（一种细胞外 Zn 螯合剂）得到挽救，表明细胞外 Zn 内流参与了神经退行性变。6-OHDA 诱导的 SNpc 中细胞内 Zn 失调（通过 ZnAF-2 荧光确定）和多巴胺能变性均通过共注射 0.25%芫荽提取物得到挽救，该提取物还减少了氨基苯荧光素荧光确定的 SNpc 中活性氧（ROS）的产生。本研究表明，芫荽叶提取物可防止由细胞内 Zn 失调引起的黑质多巴胺能神经退行性变。芫荽叶提取物的营养特性可能通过减少 ROS 产生来发挥保护作用。
