多杀巴斯德氏菌双价外膜蛋白疫苗包被于氢氧化铝纳米颗粒的免疫原性。
Immunopotential of Pasteurella multocida bivalent outer membrane protein-based vaccine entrapped in aluminum hydroxide nanoparticles.
机构信息
Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India.
Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India.
出版信息
Braz J Microbiol. 2022 Dec;53(4):2299-2307. doi: 10.1007/s42770-022-00795-1. Epub 2022 Aug 3.
Swine pasteurellosis is one of the most economically important diseases of pig caused by Pasteurella multocida (P. multocida) capsular types A and D. These organisms are commensals and opportunistic pathogens in the upper respiratory tract in pig. In the present study, we extracted whole outer membrane proteins (OMP) from P. multocida capsular types A and D and were mixed together in the ratio of 1:1 forming bivalent outer-membrane proteins. The bivalent OMP was adsorbed onto aluminum hydroxide nanoparticles. The size of aluminum hydroxide nanoparticles adsorbed outer membrane protein was found to be in the range of 125 to 130 nm. We observed that aluminum hydroxide nanoparticles adjuvanted bivalent OMP-based vaccine elicited quicker immune kinetics in terms of IgG response as compared to aluminum hydroxide microparticles adjuvanted bivalent bacterin vaccine against P. multocida capsular type A and D.
猪巴氏杆菌病是由多杀性巴氏杆菌（P. multocida）荚膜型 A 和 D 引起的一种对猪危害最严重的经济重要疾病。这些生物体在上呼吸道中是共生菌和机会性病原体。在本研究中，我们从多杀性巴氏杆菌荚膜型 A 和 D 中提取了全外膜蛋白（OMP），并以 1:1 的比例混合形成二价外膜蛋白。二价 OMP 被吸附到氢氧化铝纳米颗粒上。吸附外膜蛋白的氢氧化铝纳米颗粒的大小在 125 至 130nm 范围内。我们观察到，与氢氧化铝微球佐剂二价菌苗相比，氢氧化铝纳米颗粒佐剂二价 OMP 疫苗在 IgG 反应方面更快地引发免疫动力学，针对多杀性巴氏杆菌荚膜型 A 和 D。
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