Microbiology Lab, Department of Zoology, Government College University.
Department of Zoology, The Women University.
J Oleo Sci. 2022;71(8):1181-1188. doi: 10.5650/jos.ess22068.
Bacterial resistance to already present antibiotics demands for new approaches in field of medicine. Scientists prefer nanoparticles (NPs) due to their promising potential in many applications. Two bacterial strains, Escherichia coli and Bacillus subtilis were used for biogenic synthesis of NPs. Characterization of prepared NPs was accomplished using UV-vis spectroscopy and fourier transform infrared spectroscopy (FTIR). The prepared NPs were confirmed by the color change from pale yellow to having white deposition for Zn NPs while from dark green to light green for Ni NPs. UV-vis spectroscopy of E. coli and B. subtilis based ZnNPs showed highest peak at 354nm and 362nm, respectively. Likewise, E. coli and B. subtilis NiNPs showed peaks at 246 nm and 238 nm, respectively. Antibacterial activity of B. subtilis based ZnNPs showed significant (p ≤ 0.05) zone of inhibition (ZOI; 27.3±0.6) against B. subtilis and 26.66±0.67 against E. coli at 100 mg/mL. Antibacterial activity of E. coli based ZnNPs showed 8.3±0.3 ZOI against B. subtilis and 6.6±0.3 ZOI against E. coli while NiNPs showed (25.0±0.0 mm) (ZOI) against B. subtilis and (25.0 ± 0.3 mm) against E. coli. Minimum inhibitory concentration (MIC) of E. coli ZnNPs showed values of 6.7±0.3 μg/mL for E. coli and 4.7±0.3 μg/mL for B. subtilis. MIC of B. subtilis ZnNPs showed 5.3±0.3 μg/mL for E. coli and 6.6±0.3 μg/mL for B. subtilis while NiNPs showed 33.0±1.0 μg/mL against E. coli and 24.0±1.0 μg/mL against B. subtilis as effective inhibitory concentrations. Minimum bactericidal concentration (MBC) of E. coli ZnNPs showed 7.3±0.3 μg/mL for E. coli and 8.3±0.3 μg/mL for B. subtilis. MBC of B. subtilis ZnNPs showed 7.6±0.3 μg/mL for E. coli and 8.6±0.3 μg/mL for B. subtilis while NiNPs showed 45.7±1.3 μg/mL against E. coli and 33.0±1.0 μg/mL against B. subtilis as effective inhibitory concentrations. It was concluded from the current study that biogenically synthesized ZnNPs and NiNPs are effective as promising antibacterial agents and have potential applications in biomedical fields.
细菌对现有抗生素的耐药性要求在医学领域寻求新的方法。由于纳米粒子（NPs）在许多应用中具有有前途的潜力，因此科学家们更喜欢使用纳米粒子。使用大肠杆菌和枯草芽孢杆菌两种细菌菌株来生物合成 NPs。使用紫外-可见光谱法和傅里叶变换红外光谱法（FTIR）对制备的 NPs 进行了表征。通过 Zn NPs 的颜色从浅黄色变为白色沉淀，Ni NPs 的颜色从深绿色变为浅绿色来确认制备的 NPs。大肠杆菌和枯草芽孢杆菌的 ZnNPs 的紫外-可见光谱分别在 354nm 和 362nm 处显示出最高峰值。同样，大肠杆菌和枯草芽孢杆菌的 NiNPs 在 246nm 和 238nm 处显示出峰值。枯草芽孢杆菌基 ZnNPs 的抑菌活性对枯草芽孢杆菌表现出显著（p ≤ 0.05）抑菌区（ZOI；27.3±0.6），对大肠杆菌的抑菌活性为 26.66±0.67mg/mL。大肠杆菌基 ZnNPs 的抑菌活性对枯草芽孢杆菌的抑菌区为 8.3±0.3 ZOI，对大肠杆菌的抑菌区为 6.6±0.3 ZOI，而 NiNPs 对枯草芽孢杆菌的抑菌区为（25.0±0.0mm）（ZOI），对大肠杆菌的抑菌区为（25.0 ± 0.3mm）。大肠杆菌 ZnNPs 的最小抑菌浓度（MIC）对大肠杆菌的数值为 6.7±0.3μg/mL，对枯草芽孢杆菌的数值为 4.7±0.3μg/mL。枯草芽孢杆菌 ZnNPs 的 MIC 对大肠杆菌的数值为 5.3±0.3μg/mL，对枯草芽孢杆菌的数值为 6.6±0.3μg/mL，而 NiNPs 对大肠杆菌的数值为 33.0±1.0μg/mL，对枯草芽孢杆菌的数值为 24.0±1.0μg/mL。大肠杆菌 ZnNPs 的最小杀菌浓度（MBC）对大肠杆菌的数值为 7.3±0.3μg/mL，对枯草芽孢杆菌的数值为 8.3±0.3μg/mL。枯草芽孢杆菌 ZnNPs 的 MBC 对大肠杆菌的数值为 7.6±0.3μg/mL，对枯草芽孢杆菌的数值为 8.6±0.3μg/mL，而 NiNPs 对大肠杆菌的数值为 45.7±1.3μg/mL，对枯草芽孢杆菌的数值为 33.0±1.0μg/mL。从目前的研究中得出结论，生物合成的 ZnNPs 和 NiNPs 是有效的，具有作为有前途的抗菌剂的潜力，并具有在生物医学领域的应用潜力。
