Yigit Serbulent, Tekcan Akin, Inanir Ahmet, Nursal Ayse Feyda, Akkanat Songul, Tural Ercan
Faculty of Veterinary, Department of Genetics, Ondokuz Mayıs University, Samsun, Turkey.
Faculty of Medicine, Department of Medical Biology, Gaziosmanpasa University, Tokat, Turkey.
Nucleosides Nucleotides Nucleic Acids. 2023;42(1):65-76. doi: 10.1080/15257770.2022.2107219. Epub 2022 Aug 4.
Osteoarthritis (OA) is a complex disorder characterized by degenerative articular cartilage in which inflammatory mechanisms play a major role in the pathogenesis. Interleukin-6 (IL6), a multifunctional cytokine, can trigger osteoclast differentiation and bone resorption. Our purpose in this study was to evaluate the association of -174 G/C (rs1800795) and -572 G/C (rs1800796) variants with the susceptibility to OA. One hundred fifty OA patients and 150 healthy individuals were enrolled in the study. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used for genotyping the gene variants. The results of analyses were evaluated for statistical significance. The pain intensity was assessed using the Visual Analogue Scale (VAS). There was a statistically significant difference in the genotype and allele frequencies of the -174 G/C variant between patients with OA and control groups ( respectively). -174 G/C GG genotype and G allele were more prevalent in patients with OA. We found that the -572 G/C variant was not different between patients and controls in either genotype distribution and allele frequency. 174 G/C and -572 G/C loci GG-GG combined genotype was significantly higher in OA patients (). Our study suggests that there was a strong association between the -174 G/C variant and OA in the Turkish population. Further studies on populations of different ethnic background are necessary to prove the association of variants with OA.
骨关节炎（OA）是一种以关节软骨退变为特征的复杂疾病，其中炎症机制在发病机制中起主要作用。白细胞介素-6（IL6）是一种多功能细胞因子，可触发破骨细胞分化和骨吸收。本研究的目的是评估-174 G/C（rs1800795）和-572 G/C（rs1800796）基因变异与OA易感性的关联。本研究纳入了150例OA患者和150名健康个体。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对基因变异进行基因分型。对分析结果进行统计学意义评估。使用视觉模拟量表（VAS）评估疼痛强度。OA患者与对照组之间-174 G/C变异的基因型和等位基因频率存在统计学显著差异（分别为 ）。-174 G/C GG基因型和G等位基因在OA患者中更为普遍。我们发现，-572 G/C变异在患者和对照组之间的基因型分布和等位基因频率方面均无差异。OA患者中174 G/C和-572 G/C位点GG-GG联合基因型显著更高（ ）。我们的研究表明，在土耳其人群中，-174 G/C变异与OA之间存在强关联。有必要对不同种族背景的人群进行进一步研究，以证实基因变异与OA的关联。
