Roberts I, Smith I M
Ann Clin Biochem. 1987 Mar;24 ( Pt 2):167-71. doi: 10.1177/000456328702400207.
A simple rapid and sensitive HPLC technique for the analysis of total and free plasma indomethacin concentrations in neonates is described. The advantages of the technique are small sample volumes (50 microL) enabling the use of capillary samples, direct extraction, low limits of detection (20 ng/mL) and good precision (CV less than 7% between-batch). Indomethacin and the internal standard (flufenamic acid) eluted within 12 min and the peaks were unaffected by indomethacin metabolites or by a number of commonly used drugs. The technique is suitable for the investigation into the pharmacokinetics of total and free indomethacin, in neonates with patent ductus arteriosus, and could be used to define a therapeutic window for free indomethacin levels to enable therapeutic drug monitoring to occur in this condition.
本文描述了一种用于分析新生儿血浆中吲哚美辛总浓度和游离浓度的简单、快速且灵敏的高效液相色谱（HPLC）技术。该技术的优点包括样本量小（50微升），可使用毛细管样本，直接提取，检测限低（20纳克/毫升）以及精密度良好（批间变异系数小于7%）。吲哚美辛和内标（氟芬那酸）在12分钟内洗脱，峰不受吲哚美辛代谢物或多种常用药物的影响。该技术适用于研究患有动脉导管未闭的新生儿中总吲哚美辛和游离吲哚美辛的药代动力学，可用于确定游离吲哚美辛水平的治疗窗，以便在此情况下进行治疗药物监测。
