University of Hohenheim, Institute of Food Chemistry (170b), Garbenstraße 28, D-70599 Stuttgart, Germany.
University of Hohenheim, Institute of Crop Science, Department Physiology of Yield Stability (340k), Fruwirthstraße 21, D-70599 Stuttgart, Germany.
J Agric Food Chem. 2022 Aug 17;70(32):9856-9864. doi: 10.1021/acs.jafc.2c03374. Epub 2022 Aug 4.
Phytosterols were analyzed in 34 different quinoa accessions, which were obtained from the same field trial. Twenty different sterols were detected, and 17 could be structurally assigned by means of gas chromatography with mass spectrometry. Sterols were quantitated in selected ion monitoring mode (GC/MS-SIM) with the novel internal standard 3---butyldimethylsilyl-cholestanol (cholestanyl-TBDMS). GC/MS-SIM response factors of minor sterols were determined after enrichment by countercurrent chromatography. The total sterol contents varied from 120 to 180 mg/100 g of seeds, which is higher than has been described in quinoa before. This was due to the fact that Δ7-sterols (e.g., Δ7-sitosterol, spinasterol, and Δ7-avenasterol) were quantitated for the first time in quinoa and contributed ∼64% to the total sterol content. Clustering allowed distributing of the 34 different quinoa accessions into four distinct groups on the basis of the different sterol patterns.
对 34 种不同的藜麦品种进行了植物甾醇分析，这些品种均来自同一田间试验。检测到 20 种不同的甾醇，其中 17 种可通过气相色谱-质谱联用进行结构鉴定。甾醇采用新型内标 3-丁基二甲基硅基-胆甾醇（胆甾烷基-TBDMS）在选定离子监测模式（GC/MS-SIM）下定量。用逆流色谱法富集后，测定了少量甾醇的 GC/MS-SIM 响应因子。种子中总甾醇含量为 120-180mg/100g，高于以前描述的藜麦。这是因为首次在藜麦中定量测定了 Δ7-甾醇（如 Δ7-谷甾醇、菠菜甾醇和 Δ7-菜油甾醇），占总甾醇含量的约 64%。聚类允许根据不同的甾醇模式将 34 种不同的藜麦品种分为四个不同的组。
Zotero 插件