Willén R, Tropé C, Långström E, Ranstam J, Killander D, Clase L
Anticancer Res. 1987 Mar-Apr;7(2):235-42.
Flow cytometry was used for the investigation of the DNA distribution in biopsy specimens from 72 patients with squamous cell carcinoma of the uterine cervix. A prospective grading score system using tumor cell parameters and tumor host parameters was also applied. 50% of the tumors were aneuploid with up to 4 different tumor populations. The definition of DI +/- 8% was applied. The median age of the patients was 61 years with FIGO median value of 2B. Significant correlations were observed between ploidy and MGS-scores and stage stadium according to FIGO. Increasing MGS score was noted with increasingly distorted ploidy. No significant difference was found between DI below and above 1.5 for MGS, FIGO stage stadium, histology according to Ackerman, tumor parameters and tumor host parameters for the total material. However, for aneuploid tumors MGS and tumor cell parameters were related with DI below and above 1.5 (P = 0.05 and P = 0.02, respectively). No correlation between clinical stage according to FIGO and S-phase % was noted. It remains to be settled to what extent DNA flow cytometry and MGS-scoring in our ongoing prospective series of invasive squamous cell carcinoma of the uterine cervix can take their place in the multifactorial prognostic index suggested by Jacobsen et al (Am. J. Clin. Oncol. 8: 39, 1985).
采用流式细胞术对72例子宫颈鳞状细胞癌患者活检标本中的DNA分布进行研究。还应用了一种使用肿瘤细胞参数和肿瘤宿主参数的前瞻性分级评分系统。50%的肿瘤为非整倍体，存在多达4种不同的肿瘤群体。采用DI +/- 8%的定义。患者的中位年龄为61岁，国际妇产科联盟（FIGO）分期的中位值为2B期。观察到倍体与MGS评分以及根据FIGO分期之间存在显著相关性。随着倍体畸变程度增加，MGS评分也升高。对于整个研究材料，MGS、FIGO分期、根据阿克曼分类的组织学、肿瘤参数和肿瘤宿主参数在DI低于和高于1.5时均未发现显著差异。然而，对于非整倍体肿瘤，MGS和肿瘤细胞参数与DI低于和高于1.5相关（分别为P = 0.05和P = 0.02）。未观察到根据FIGO的临床分期与S期百分比之间存在相关性。在我们正在进行的子宫颈浸润性鳞状细胞癌前瞻性系列研究中，DNA流式细胞术和MGS评分在多大程度上能够在雅各布森等人（《美国临床肿瘤学杂志》8: 39, 1985）提出的多因素预后指数中发挥作用，仍有待确定。
