从 中克隆和表达 SAK 酶于 BL21-CodonPlus。
Cloning & expression of SAK enzyme from in BL21-CodonPlus.
机构信息
Ministry of Higher Education and Scientific Research, Baghdad, Iraq.
Pharmacy Department, AL-Huda University College, Al Anbar, Iraq.
出版信息
J Med Life. 2022 Jun;15(6):768-771. doi: 10.25122/jml-2021-0335.
Staphylokinase (SAK), also known as staphylococcal fibrinolysin, is a protein with a molecular mass of about 15 kDa produced by . Staphylokinase is synthesized in the late exponential phase, similar to streptokinase. The current study identified and predicted the protein SAK from . SAK is a fibrinolytic enzyme of the third generation that acts as an indirect activator of plasminogen. The current study cloned and expressed SAK protein isolated from and used in the form of a grid for enhancement of SAK Catalyst with PCR, disengagement, and change into articulation vector PET24b(+). The recombinant plasmid was changed into strain BL21 (codon additionally to 440) acceptance with isopropyl β-D-1-thiogalactopyranoside (IPTG).
葡萄球菌激酶（SAK），也称为金葡菌纤维蛋白溶酶，是一种分子量约为 15 kDa 的蛋白质，由 产生。SAK 与链激酶相似，在指数晚期合成。本研究从 中鉴定和预测了蛋白 SAK。SAK 是第三代纤溶酶，作为纤溶酶原的间接激活剂。本研究克隆并表达了从 中分离的 SAK 蛋白，并以网格形式用于增强 SAK 用 PCR、分离和转化为表达载体 PET24b(+)。将重组质粒转化为 BL21 菌株（密码子额外添加到 440）接受异丙基 β-D-1-硫代半乳糖苷（IPTG）。
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