Guo Qinghui, Peng Yankun, Chao Kuanglin
College of Engineering, National R&D Center for Agro-processing Equipment, China Agricultural University, Beijing 100083, China.
USDA/ARS Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Heliyon. 2022 May 30;8(6):e09576. doi: 10.1016/j.heliyon.2022.e09576. eCollection 2022 Jun.
Salbutamol is a -adrenergic receptor agonist compound which has been abused as an animal growth promoter to improve carcass lean meat percentage. At present, the detection of salbutamol by SERS mostly uses gold colloid as substrate, which is expensive and has a high detection limit. In this report, Raman enhancement signal of salbutamol was compared with concentrated gold and silver colloids. The results show that the concentrated silver colloid prepared by reducing silver nitrate with hydroxylamine hydrochloride had superior performance. Three silver colloids with different particle sizes were synthesized by the same reducing agent and used as substrates for spectra acquisition of salbutamol to explore the enhancement performance of different silver nanoparticles sizes on salbutamol. The results showed that silver nanoparticles with larger particle sizes were more conducive to the adsorption of salbutamol. Finally, under the optimal conditions (Silver colloid A as enhanced substrate, 0.2 mol/L NaOH aqueous solution as aggregating compound), a better linear relationship between the concentration of salbutamol (ranged from 0.2 to 1 mg/L) and SERS intensity. The linear equation between SERS intensity and salbutamol concentration was = 0.0023-0.079 (mg/L) with a good linearity ( =0.994) and lower root mean square error ( = 0.022 mg/L), where (mg/L) was the concentration of salbutamol solution and was the SERS intensity of salbutamol solution. Validation set correlation coefficient was 0.988 and prediction root mean square error was 0.029 mg/L. This method provides a new idea for further reducing the detection limit of salbutamol. This study is helpful to further develop a simple and low-cost SERS detection method of salbutamol based on silver colloid.
沙丁胺醇是一种β-肾上腺素能受体激动剂化合物，曾被滥用作动物生长促进剂以提高胴体瘦肉率。目前，表面增强拉曼光谱（SERS）法检测沙丁胺醇大多使用金胶体作为底物，成本高且检测限高。本报告将沙丁胺醇的拉曼增强信号与浓金胶体和浓银胶体进行了比较。结果表明，用盐酸羟胺还原硝酸银制备的浓银胶体性能更优。用同一还原剂合成了三种不同粒径的银胶体，并将其用作沙丁胺醇光谱采集的底物，以探究不同粒径的银纳米颗粒对沙丁胺醇的增强性能。结果表明，粒径较大的银纳米颗粒更有利于沙丁胺醇的吸附。最后，在最佳条件下（以银胶体A为增强底物，0.2 mol/L氢氧化钠水溶液为聚集剂），沙丁胺醇浓度（范围为0.2至1 mg/L）与SERS强度之间呈现出较好的线性关系。SERS强度与沙丁胺醇浓度的线性方程为 = 0.0023 - 0.079（mg/L），线性良好（ = 0.994）且均方根误差较低（ = 0.022 mg/L），其中 （mg/L）为沙丁胺醇溶液浓度， 为沙丁胺醇溶液的SERS强度。验证集相关系数为0.988，预测均方根误差为0.029 mg/L。该方法为进一步降低沙丁胺醇的检测限提供了新思路。本研究有助于进一步开发基于银胶体的简单、低成本的沙丁胺醇SERS检测方法。
